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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio vulnificus Cytochrome c-552 (nrfA) CSB-YP742096VCQ
CSB-EP742096VCQ
CSB-BP742096VCQ
CSB-MP742096VCQ
CSB-EP742096VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP742097VCQ
CSB-EP742097VCQ
CSB-BP742097VCQ
CSB-MP742097VCQ
CSB-EP742097VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribonuclease T (rnt) CSB-YP742098VCQ
CSB-EP742098VCQ
CSB-BP742098VCQ
CSB-MP742098VCQ
CSB-EP742098VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0061 protein VV1089 (VV1089) CSB-YP742099VCQ
CSB-EP742099VCQ
CSB-BP742099VCQ
CSB-MP742099VCQ
CSB-EP742099VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Glutamine--tRNA ligase (glnS), partial CSB-YP742100VCQ
CSB-EP742100VCQ
CSB-BP742100VCQ
CSB-MP742100VCQ
CSB-EP742100VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Peptide chain release factor 1 (prfA) CSB-YP742101VCQ
CSB-EP742101VCQ
CSB-BP742101VCQ
CSB-MP742101VCQ
CSB-EP742101VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Probable rRNA maturation factor (VV0915) CSB-YP742102VCQ
CSB-EP742102VCQ
CSB-BP742102VCQ
CSB-MP742102VCQ
CSB-EP742102VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein Xni (xni) CSB-YP742103VCQ
CSB-EP742103VCQ
CSB-BP742103VCQ
CSB-MP742103VCQ
CSB-EP742103VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Transcriptional repressor NrdR (nrdR) CSB-YP742104VCQ
CSB-EP742104VCQ
CSB-BP742104VCQ
CSB-MP742104VCQ
CSB-EP742104VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Membrane-bound lytic murein transglycosylase F (mltF), partial CSB-YP742105VCQ
CSB-EP742105VCQ
CSB-BP742105VCQ
CSB-MP742105VCQ
CSB-EP742105VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus D-amino acid dehydrogenase small subunit (dadA) CSB-YP742106VCQ
CSB-EP742106VCQ
CSB-BP742106VCQ
CSB-MP742106VCQ
CSB-EP742106VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP742107VCQ
CSB-EP742107VCQ
CSB-BP742107VCQ
CSB-MP742107VCQ
CSB-EP742107VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP742108VCQ
CSB-EP742108VCQ
CSB-BP742108VCQ
CSB-MP742108VCQ
CSB-EP742108VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Trp operon repressor homolog (trpR) CSB-YP742109VCQ
CSB-EP742109VCQ
CSB-BP742109VCQ
CSB-MP742109VCQ
CSB-EP742109VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus RNA pyrophosphohydrolase (rppH) CSB-YP742110VCQ
CSB-EP742110VCQ
CSB-BP742110VCQ
CSB-MP742110VCQ
CSB-EP742110VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Probable endonuclease 4 (nfo) CSB-YP742111VCQ
CSB-EP742111VCQ
CSB-BP742111VCQ
CSB-MP742111VCQ
CSB-EP742111VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Dihydrodipicolinate reductase (dapB) CSB-YP742112VCQ
CSB-EP742112VCQ
CSB-BP742112VCQ
CSB-MP742112VCQ
CSB-EP742112VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP742113VCQ
CSB-EP742113VCQ
CSB-BP742113VCQ
CSB-MP742113VCQ
CSB-EP742113VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Undecaprenyl-diphosphatase (uppP), partial CSB-YP742114VCQ
CSB-EP742114VCQ
CSB-BP742114VCQ
CSB-MP742114VCQ
CSB-EP742114VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP742115VCQ
CSB-EP742115VCQ
CSB-BP742115VCQ
CSB-MP742115VCQ
CSB-EP742115VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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