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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Lactobacillus johnsonii Elongation factor G (fusA), partial CSB-YP741555LAW
CSB-EP741555LAW
CSB-BP741555LAW
CSB-MP741555LAW
CSB-EP741555LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP741556LAW
CSB-EP741556LAW
CSB-BP741556LAW
CSB-MP741556LAW
CSB-EP741556LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP741557LAW
CSB-EP741557LAW
CSB-BP741557LAW
CSB-MP741557LAW
CSB-EP741557LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii D-alanine--D-alanine ligase (ddl) CSB-YP741558LAW
CSB-EP741558LAW
CSB-BP741558LAW
CSB-MP741558LAW
CSB-EP741558LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii DNA replication and repair protein recF (recF) CSB-YP741559LAW
CSB-EP741559LAW
CSB-BP741559LAW
CSB-MP741559LAW
CSB-EP741559LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans Protein NEQ441 (NEQ441) CSB-YP741560NAQ
CSB-EP741560NAQ
CSB-BP741560NAQ
CSB-MP741560NAQ
CSB-EP741560NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans DNA-directed RNA polymerase subunit N (rpoN) CSB-YP741561NAQ
CSB-EP741561NAQ
CSB-BP741561NAQ
CSB-MP741561NAQ
CSB-EP741561NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans 30S ribosomal protein S10P (rps10p) CSB-YP741562NAQ
CSB-EP741562NAQ
CSB-BP741562NAQ
CSB-MP741562NAQ
CSB-EP741562NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans Threonine--tRNA ligase (thrS), partial CSB-YP741563NAQ
CSB-EP741563NAQ
CSB-BP741563NAQ
CSB-MP741563NAQ
CSB-EP741563NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans 50S ribosomal protein L29P (rpl29p) CSB-YP741564NAQ
CSB-EP741564NAQ
CSB-BP741564NAQ
CSB-MP741564NAQ
CSB-EP741564NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans DNA repair and recombination protein radA (radA) CSB-YP741565NAQ
CSB-EP741565NAQ
CSB-BP741565NAQ
CSB-MP741565NAQ
CSB-EP741565NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans 50S ribosomal protein L21e (rpl21e) CSB-YP741566NAQ
CSB-EP741566NAQ
CSB-BP741566NAQ
CSB-MP741566NAQ
CSB-EP741566NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans 50S ribosomal protein L14P (rpl14p) CSB-YP741567NAQ
CSB-EP741567NAQ
CSB-BP741567NAQ
CSB-MP741567NAQ
CSB-EP741567NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans Proline--tRNA ligase (proS), partial CSB-YP741568NAQ
CSB-EP741568NAQ
CSB-BP741568NAQ
CSB-MP741568NAQ
CSB-EP741568NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Plasmid replication protein RepX (repX) CSB-YP741569BQN
CSB-EP741569BQN
CSB-BP741569BQN
CSB-MP741569BQN
CSB-EP741569BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DnaJ-like protein DjlA (djlA), partial CSB-YP741570YAS
CSB-EP741570YAS
CSB-BP741570YAS
CSB-MP741570YAS
CSB-EP741570YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0325 protein YPO1040/y3141/YP_2811 (YPO1040, y3141, YP_2811) CSB-YP741571YAS
CSB-EP741571YAS
CSB-BP741571YAS
CSB-MP741571YAS
CSB-EP741571YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein ClpB (clpB), partial CSB-YP741572YAS
CSB-EP741572YAS
CSB-BP741572YAS
CSB-MP741572YAS
CSB-EP741572YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DNA replication complex GINS protein PSF3 (PSF3) CSB-YP741573DOT
CSB-EP741573DOT
CSB-BP741573DOT
CSB-MP741573DOT
CSB-EP741573DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Diphthamide biosynthesis protein 3 (DPH3) CSB-YP741574DOT
CSB-EP741574DOT
CSB-BP741574DOT
CSB-MP741574DOT
CSB-EP741574DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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