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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Ferredoxin--NADP reductase (SA2162) CSB-YP741815SKY
CSB-EP741815SKY
CSB-BP741815SKY
CSB-MP741815SKY
CSB-EP741815SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lysostaphin resistance protein A (lyrA), partial CSB-YP741816SKY
CSB-EP741816SKY
CSB-BP741816SKY
CSB-MP741816SKY
CSB-EP741816SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urease accessory protein UreE (ureE) CSB-YP741817SKY
CSB-EP741817SKY
CSB-BP741817SKY
CSB-MP741817SKY
CSB-EP741817SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L14 (rplN) CSB-YP741818SKY
CSB-EP741818SKY
CSB-BP741818SKY
CSB-MP741818SKY
CSB-EP741818SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Multidrug resistance efflux pump sepA (sepA), partial CSB-YP741819SKY1
CSB-EP741819SKY1
CSB-BP741819SKY1
CSB-MP741819SKY1
CSB-EP741819SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit a (atpB), partial CSB-YP741820SKY1
CSB-EP741820SKY1
CSB-BP741820SKY1
CSB-MP741820SKY1
CSB-EP741820SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus L-threonine dehydratase biosynthetic IlvA (ilvA) CSB-YP741821SKY
CSB-EP741821SKY
CSB-BP741821SKY
CSB-MP741821SKY
CSB-EP741821SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized RNA methyltransferase SA1713 (SA1713) CSB-YP741822SKY
CSB-EP741822SKY
CSB-BP741822SKY
CSB-MP741822SKY
CSB-EP741822SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamate-1-semialdehyde 2,1-aminomutase 2 (hemL2) CSB-YP741823SKY
CSB-EP741823SKY
CSB-BP741823SKY
CSB-MP741823SKY
CSB-EP741823SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease splF (splF) CSB-YP741824SKY
CSB-EP741824SKY
CSB-BP741824SKY
CSB-MP741824SKY
CSB-EP741824SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tyrosine--tRNA ligase (tyrS) CSB-YP741825SKY
CSB-EP741825SKY
CSB-BP741825SKY
CSB-MP741825SKY
CSB-EP741825SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable cell wall amidase LytH (lytH) CSB-YP741826SKY
CSB-EP741826SKY
CSB-BP741826SKY
CSB-MP741826SKY
CSB-EP741826SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcriptional regulatory protein srrA (srrA) CSB-YP741827SKY
CSB-EP741827SKY
CSB-BP741827SKY
CSB-MP741827SKY
CSB-EP741827SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0398 protein SA1280 (SA1280) CSB-YP741828SKY
CSB-EP741828SKY
CSB-BP741828SKY
CSB-MP741828SKY
CSB-EP741828SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0346 protein SA1254 (SA1254) CSB-YP741829SKY
CSB-EP741829SKY
CSB-BP741829SKY
CSB-MP741829SKY
CSB-EP741829SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphate-binding protein pstS (pstS) CSB-YP741830SKY
CSB-EP741830SKY
CSB-BP741830SKY
CSB-MP741830SKY
CSB-EP741830SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein GlcT (glcT) CSB-YP741831SKY
CSB-EP741831SKY
CSB-BP741831SKY
CSB-MP741831SKY
CSB-EP741831SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease J 2 (SA1118), partial CSB-YP741832SKY
CSB-EP741832SKY
CSB-BP741832SKY
CSB-MP741832SKY
CSB-EP741832SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP741833SKY
CSB-EP741833SKY
CSB-BP741833SKY
CSB-MP741833SKY
CSB-EP741833SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable heme-iron transport system permease protein IsdF (isdF), partial CSB-YP741834SKY
CSB-EP741834SKY
CSB-BP741834SKY
CSB-MP741834SKY
CSB-EP741834SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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