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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Macrolide export ATP-binding/permease protein MacB 2 (macB2), partial CSB-YP741875YAS1
CSB-EP741875YAS1
CSB-BP741875YAS1
CSB-MP741875YAS1
CSB-EP741875YAS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant LPS-assembly lipoprotein lptE (lptE) CSB-YP741876YAS
CSB-EP741876YAS
CSB-BP741876YAS
CSB-MP741876YAS
CSB-EP741876YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP741877YAS
CSB-EP741877YAS
CSB-BP741877YAS
CSB-MP741877YAS
CSB-EP741877YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-ribose pyranase (rbsD) CSB-YP741878YAS
CSB-EP741878YAS
CSB-BP741878YAS
CSB-MP741878YAS
CSB-EP741878YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0337 protein spyM18_2071 (spyM18_2071) CSB-YP741879SMU
CSB-EP741879SMU
CSB-BP741879SMU
CSB-MP741879SMU
CSB-EP741879SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine regulator (argR) CSB-YP741880SMU
CSB-EP741880SMU
CSB-BP741880SMU
CSB-MP741880SMU
CSB-EP741880SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit a (atpB), partial CSB-YP741881SMU1
CSB-EP741881SMU1
CSB-BP741881SMU1
CSB-MP741881SMU1
CSB-EP741881SMU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L6 (rplF) CSB-YP741882SMU
CSB-EP741882SMU
CSB-BP741882SMU
CSB-MP741882SMU
CSB-EP741882SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Multidrug resistance protein MdtM (mdtM), partial CSB-YP741883SXB
CSB-EP741883SXB
CSB-BP741883SXB
CSB-MP741883SXB
CSB-EP741883SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP741884SXB1
CSB-EP741884SXB1
CSB-BP741884SXB1
CSB-MP741884SXB1
CSB-EP741884SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium ATP synthase subunit a (atpB), partial CSB-YP741885SXB1
CSB-EP741885SXB1
CSB-BP741885SXB1
CSB-MP741885SXB1
CSB-EP741885SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Ferrous iron transport protein C (feoC) CSB-YP741886SXB
CSB-EP741886SXB
CSB-BP741886SXB
CSB-MP741886SXB
CSB-EP741886SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA), partial CSB-YP741887SXB1
CSB-EP741887SXB1
CSB-BP741887SXB1
CSB-MP741887SXB1
CSB-EP741887SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium G/U mismatch-specific DNA glycosylase (mug) CSB-YP741888SXB
CSB-EP741888SXB
CSB-BP741888SXB
CSB-MP741888SXB
CSB-EP741888SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Transcriptional repressor rcnR (rcnR) CSB-YP741889SXB
CSB-EP741889SXB
CSB-BP741889SXB
CSB-MP741889SXB
CSB-EP741889SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium DnaA-homolog protein hda (hda) CSB-YP741890SXB
CSB-EP741890SXB
CSB-BP741890SXB
CSB-MP741890SXB
CSB-EP741890SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0352 protein yejL (yejL) CSB-YP741891SXB
CSB-EP741891SXB
CSB-BP741891SXB
CSB-MP741891SXB
CSB-EP741891SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Thymidine kinase (tdk) CSB-YP741892SXB
CSB-EP741892SXB
CSB-BP741892SXB
CSB-MP741892SXB
CSB-EP741892SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0502 protein YceH (yceH) CSB-YP741893SXB
CSB-EP741893SXB
CSB-BP741893SXB
CSB-MP741893SXB
CSB-EP741893SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Glutathione transport system permease protein gsiD (gsiD), partial CSB-YP741894SXB1
CSB-EP741894SXB1
CSB-BP741894SXB1
CSB-MP741894SXB1
CSB-EP741894SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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