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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Multidrug resistance protein MdtH (mdtH), partial CSB-YP741855EOD
CSB-EP741855EOD
CSB-BP741855EOD
CSB-MP741855EOD
CSB-EP741855EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia pneumoniae Serine/threonine-protein kinase pkn1 (pkn1), partial CSB-YP741856DRZ
CSB-EP741856DRZ
CSB-BP741856DRZ
CSB-MP741856DRZ
CSB-EP741856DRZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Type I secretion system ATP-binding protein PrsD (prsD), partial CSB-YP741857RKU1
CSB-EP741857RKU1
CSB-BP741857RKU1
CSB-MP741857RKU1
CSB-EP741857RKU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a UPF0473 protein lin1536 (lin1536) CSB-YP741858LGU
CSB-EP741858LGU
CSB-BP741858LGU
CSB-MP741858LGU
CSB-EP741858LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional AAC/APH (aacA-aphD) CSB-YP741859SUO
CSB-EP741859SUO
CSB-BP741859SUO
CSB-MP741859SUO
CSB-EP741859SUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guanine nucleotide exchange factor sopE (sopE) CSB-YP741860SFAZ
CSB-EP741860SFAZ
CSB-BP741860SFAZ
CSB-MP741860SFAZ
CSB-EP741860SFAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkyl hydroperoxide reductase subunit C (ahpC) CSB-YP741861MVZ
CSB-EP741861MVZ
CSB-BP741861MVZ
CSB-MP741861MVZ
CSB-EP741861MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein impA (impA) CSB-YP741862SZB
CSB-EP741862SZB
CSB-BP741862SZB
CSB-MP741862SZB
CSB-EP741862SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Secreted effector protein SseB (sseB) CSB-YP741863SXB
CSB-EP741863SXB
CSB-BP741863SXB
CSB-MP741863SXB
CSB-EP741863SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Xylose isomerase (xylA) CSB-YP741864ELW
CSB-EP741864ELW
CSB-BP741864ELW
CSB-MP741864ELW
CSB-EP741864ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Protein GlcT (glcT) CSB-YP741865SLD
CSB-EP741865SLD
CSB-BP741865SLD
CSB-MP741865SLD
CSB-EP741865SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylose import ATP-binding protein XylG (xylG), partial CSB-YP741866YAS
CSB-EP741866YAS
CSB-BP741866YAS
CSB-MP741866YAS
CSB-EP741866YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L14 (rplN) CSB-YP741867YAS
CSB-EP741867YAS
CSB-BP741867YAS
CSB-MP741867YAS
CSB-EP741867YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP741868YAS
CSB-EP741868YAS
CSB-BP741868YAS
CSB-MP741868YAS
CSB-EP741868YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein FtsP (ftsP) CSB-YP741869YAS
CSB-EP741869YAS
CSB-BP741869YAS
CSB-MP741869YAS
CSB-EP741869YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 8-amino-7-oxononanoate synthase (bioF) CSB-YP741870YAS
CSB-EP741870YAS
CSB-BP741870YAS
CSB-MP741870YAS
CSB-EP741870YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP741871YAS
CSB-EP741871YAS
CSB-BP741871YAS
CSB-MP741871YAS
CSB-EP741871YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N-acetylneuraminate epimerase (nanM) CSB-YP741872YAS
CSB-EP741872YAS
CSB-BP741872YAS
CSB-MP741872YAS
CSB-EP741872YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyridoxamine kinase (pdxY) CSB-YP741873YAS
CSB-EP741873YAS
CSB-BP741873YAS
CSB-MP741873YAS
CSB-EP741873YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH) CSB-YP741874YAS
CSB-EP741874YAS
CSB-BP741874YAS
CSB-MP741874YAS
CSB-EP741874YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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