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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Putative aldehyde dehydrogenase SA1924 (SA1924) CSB-YP748564SKY
CSB-EP748564SKY
CSB-BP748564SKY
CSB-MP748564SKY
CSB-EP748564SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative thiaminase-2 (tenA) CSB-YP748565SKY
CSB-EP748565SKY
CSB-BP748565SKY
CSB-MP748565SKY
CSB-EP748565SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized protein SA1737 (SA1737) CSB-YP748566SKY
CSB-EP748566SKY
CSB-BP748566SKY
CSB-MP748566SKY
CSB-EP748566SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ferritin (ftnA) CSB-YP748567SKY
CSB-EP748567SKY
CSB-BP748567SKY
CSB-MP748567SKY
CSB-EP748567SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peroxide-responsive repressor perR (perR) CSB-YP748568SKY
CSB-EP748568SKY
CSB-BP748568SKY
CSB-MP748568SKY
CSB-EP748568SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease splC (splC) CSB-YP748569SKY
CSB-EP748569SKY
CSB-BP748569SKY
CSB-MP748569SKY
CSB-EP748569SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0478 protein SA1560 (SA1560), partial CSB-YP748570SKY
CSB-EP748570SKY
CSB-BP748570SKY
CSB-MP748570SKY
CSB-EP748570SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTPase obg (obg) CSB-YP748571SKY
CSB-EP748571SKY
CSB-BP748571SKY
CSB-MP748571SKY
CSB-EP748571SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Pyrroline-5-carboxylate reductase (proC) CSB-YP748572SKY
CSB-EP748572SKY
CSB-BP748572SKY
CSB-MP748572SKY
CSB-EP748572SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cell cycle protein GpsB (gpsB) CSB-YP748573SKY
CSB-EP748573SKY
CSB-BP748573SKY
CSB-MP748573SKY
CSB-EP748573SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (odhB) CSB-YP748574SKY
CSB-EP748574SKY
CSB-BP748574SKY
CSB-MP748574SKY
CSB-EP748574SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Conserved virulence factor B (cvfB) CSB-YP748575SKY
CSB-EP748575SKY
CSB-BP748575SKY
CSB-MP748575SKY
CSB-EP748575SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphatidylglycerol lysyltransferase (mprF), partial CSB-YP748576SKY
CSB-EP748576SKY
CSB-BP748576SKY
CSB-MP748576SKY
CSB-EP748576SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S15 (rpsO) CSB-YP748577SKY
CSB-EP748577SKY
CSB-BP748577SKY
CSB-MP748577SKY
CSB-EP748577SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cell division protein SepF (sepF) CSB-YP748578SKY
CSB-EP748578SKY
CSB-BP748578SKY
CSB-MP748578SKY
CSB-EP748578SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Iron-regulated surface determinant protein C (isdC) CSB-YP748579SKY
CSB-EP748579SKY
CSB-BP748579SKY
CSB-MP748579SKY
CSB-EP748579SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0358 protein SA0961 (SA0961) CSB-YP748580SKY
CSB-EP748580SKY
CSB-BP748580SKY
CSB-MP748580SKY
CSB-EP748580SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable quinol oxidase subunit 3 (qoxC), partial CSB-YP748581SKY1
CSB-EP748581SKY1
CSB-BP748581SKY1
CSB-MP748581SKY1
CSB-EP748581SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease htrA-like (SA0879), partial CSB-YP748582SKY1
CSB-EP748582SKY1
CSB-BP748582SKY1
CSB-MP748582SKY1
CSB-EP748582SKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Coenzyme A disulfide reductase (cdr) CSB-YP748583SKY
CSB-EP748583SKY
CSB-BP748583SKY
CSB-MP748583SKY
CSB-EP748583SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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