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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant sn-glycerol-3-phosphate transport system permease protein ugpE (ugpE), partial CSB-YP748624YAS1
CSB-EP748624YAS1
CSB-BP748624YAS1
CSB-MP748624YAS1
CSB-EP748624YAS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris UDP-glucuronate:glycolipid 2-beta-glucuronosyltransferase (gumK) CSB-YP748625XAY
CSB-EP748625XAY
CSB-BP748625XAY
CSB-MP748625XAY
CSB-EP748625XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Energy-coupling factor transporter ATP-binding protein EcfA 1 (ecfA1) CSB-YP748626SMU
CSB-EP748626SMU
CSB-BP748626SMU
CSB-MP748626SMU
CSB-EP748626SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional protein FolD (folD) CSB-YP748627SMU
CSB-EP748627SMU
CSB-BP748627SMU
CSB-MP748627SMU
CSB-EP748627SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0346 protein spyM18_0510 (spyM18_0510) CSB-YP748628SMU
CSB-EP748628SMU
CSB-BP748628SMU
CSB-MP748628SMU
CSB-EP748628SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L14 (rplN) CSB-YP748629SMU
CSB-EP748629SMU
CSB-BP748629SMU
CSB-MP748629SMU
CSB-EP748629SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Urease subunit beta 1 (ureB1) CSB-YP748630BMO
CSB-EP748630BMO
CSB-BP748630BMO
CSB-MP748630BMO
CSB-EP748630BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Cell cycle response regulator CtrA (ctrA) CSB-YP748631BMO
CSB-EP748631BMO
CSB-BP748631BMO
CSB-MP748631BMO
CSB-EP748631BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Enamine/imine deaminase (ridA) CSB-YP748632SXB
CSB-EP748632SXB
CSB-BP748632SXB
CSB-MP748632SXB
CSB-EP748632SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0438 protein yifE (yifE) CSB-YP748633SXB
CSB-EP748633SXB
CSB-BP748633SXB
CSB-MP748633SXB
CSB-EP748633SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Purine ribonucleoside efflux pump nepI (nepI), partial CSB-YP748634SXB
CSB-EP748634SXB
CSB-BP748634SXB
CSB-MP748634SXB
CSB-EP748634SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 50S ribosomal protein L14 (rplN) CSB-YP748635SXB
CSB-EP748635SXB
CSB-BP748635SXB
CSB-MP748635SXB
CSB-EP748635SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium D-tagatose-1,6-bisphosphate aldolase subunit GatY (gatY) CSB-YP748636SXB
CSB-EP748636SXB
CSB-BP748636SXB
CSB-MP748636SXB
CSB-EP748636SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0350 protein ygfY (ygfY) CSB-YP748637SXB
CSB-EP748637SXB
CSB-BP748637SXB
CSB-MP748637SXB
CSB-EP748637SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Autonomous glycyl radical cofactor (grcA) CSB-YP748638SXB
CSB-EP748638SXB
CSB-BP748638SXB
CSB-MP748638SXB
CSB-EP748638SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Flagellar regulator flk (flk), partial CSB-YP748639SXB
CSB-EP748639SXB
CSB-BP748639SXB
CSB-MP748639SXB
CSB-EP748639SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 1,4-Dihydroxy-2-naphthoyl-CoA synthase (menB) CSB-YP748640SXB
CSB-EP748640SXB
CSB-BP748640SXB
CSB-MP748640SXB
CSB-EP748640SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Stationary-phase-induced ribosome-associated protein (sra) CSB-YP748641SXB
CSB-EP748641SXB
CSB-BP748641SXB
CSB-MP748641SXB
CSB-EP748641SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Tyrosine--tRNA ligase (tyrS) CSB-YP748642SXB
CSB-EP748642SXB
CSB-BP748642SXB
CSB-MP748642SXB
CSB-EP748642SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0434 protein ycaR (ycaR) CSB-YP748643SXB
CSB-EP748643SXB
CSB-BP748643SXB
CSB-MP748643SXB
CSB-EP748643SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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