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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Probable acetyl-CoA acyltransferase (SAR0351) CSB-YP750166SKV
CSB-EP750166SKV
CSB-BP750166SKV
CSB-MP750166SKV
CSB-EP750166SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein essB (essB), partial CSB-YP750167SKV
CSB-EP750167SKV
CSB-BP750167SKV
CSB-MP750167SKV
CSB-EP750167SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein esaA (esaA), partial CSB-YP750168SKV
CSB-EP750168SKV
CSB-BP750168SKV
CSB-MP750168SKV
CSB-EP750168SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 (ispD2) CSB-YP750169SKV
CSB-EP750169SKV
CSB-BP750169SKV
CSB-MP750169SKV
CSB-EP750169SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus PTS system EIIBC component SAR0193 (SAR0193), partial CSB-YP750170SKV
CSB-EP750170SKV
CSB-BP750170SKV
CSB-MP750170SKV
CSB-EP750170SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heme-degrading monooxygenase isdI (isdI) CSB-YP750171SKV
CSB-EP750171SKV
CSB-BP750171SKV
CSB-MP750171SKV
CSB-EP750171SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Diacetyl reductase [ (S)-acetoin forming] (butA) CSB-YP750172SKV
CSB-EP750172SKV
CSB-BP750172SKV
CSB-MP750172SKV
CSB-EP750172SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable tRNA-dihydrouridine synthase (dus) CSB-YP750173SKV
CSB-EP750173SKV
CSB-BP750173SKV
CSB-MP750173SKV
CSB-EP750173SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA gyrase subunit B (gyrB), partial CSB-YP750174SKV
CSB-EP750174SKV
CSB-BP750174SKV
CSB-MP750174SKV
CSB-EP750174SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Serine/threonine-protein phosphatase PGAM5, mitochondrial (pgam5), partial CSB-YP750175XBF
CSB-EP750175XBF
CSB-BP750175XBF
CSB-MP750175XBF
CSB-EP750175XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis DNA-binding protein inhibitor ID-4 (id4) CSB-YP750176XBF
CSB-EP750176XBF
CSB-BP750176XBF
CSB-MP750176XBF
CSB-EP750176XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Dihydropyrimidinase-related protein 3 (dpysl3), partial CSB-YP750177XBF
CSB-EP750177XBF
CSB-BP750177XBF
CSB-MP750177XBF
CSB-EP750177XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Homeobox protein EMX1 (emx1) CSB-YP750178XBF
CSB-EP750178XBF
CSB-BP750178XBF
CSB-MP750178XBF
CSB-EP750178XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Ubiquitin-like modifier-activating enzyme 5 (uba5) CSB-YP750179XBF
CSB-EP750179XBF
CSB-BP750179XBF
CSB-MP750179XBF
CSB-EP750179XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Acyl-CoA synthetase family member 3, mitochondrial (acsf3), partial CSB-YP750180XBE
CSB-EP750180XBE
CSB-BP750180XBE
CSB-MP750180XBE
CSB-EP750180XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Serpin B5 (serpinb5) CSB-YP750181XBE
CSB-EP750181XBE
CSB-BP750181XBE
CSB-MP750181XBE
CSB-EP750181XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Regulator of G-protein signaling 9-binding protein C (rgs9bp-c), partial CSB-YP750182XBE
CSB-EP750182XBE
CSB-BP750182XBE
CSB-MP750182XBE
CSB-EP750182XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Growth hormone-regulated TBC protein 1 (grtp1) CSB-YP750183XBE
CSB-EP750183XBE
CSB-BP750183XBE
CSB-MP750183XBE
CSB-EP750183XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Septin-7 (41524) CSB-YP750184XBE
CSB-EP750184XBE
CSB-BP750184XBE
CSB-MP750184XBE
CSB-EP750184XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase MARCH5 (41338), partial CSB-YP750185XBE1
CSB-EP750185XBE1
CSB-BP750185XBE1
CSB-MP750185XBE1
CSB-EP750185XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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