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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bartonella quintana 50S ribosomal protein L31 (rpmE) CSB-YP749885BSH
CSB-EP749885BSH
CSB-BP749885BSH
CSB-MP749885BSH
CSB-EP749885BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP749886BSH
CSB-EP749886BSH
CSB-BP749886BSH
CSB-MP749886BSH
CSB-EP749886BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Type IV secretion system protein virB8 (virB8), partial CSB-YP749887BSH
CSB-EP749887BSH
CSB-BP749887BSH
CSB-MP749887BSH
CSB-EP749887BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Glycine cleavage system H protein (gcvH) CSB-YP749888BSH
CSB-EP749888BSH
CSB-BP749888BSH
CSB-MP749888BSH
CSB-EP749888BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP749889BSH
CSB-EP749889BSH
CSB-BP749889BSH
CSB-MP749889BSH
CSB-EP749889BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Ketol-acid reductoisomerase (ilvC) CSB-YP749890BSH
CSB-EP749890BSH
CSB-BP749890BSH
CSB-MP749890BSH
CSB-EP749890BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana 50S ribosomal protein L4 (rplD) CSB-YP749891BSH
CSB-EP749891BSH
CSB-BP749891BSH
CSB-MP749891BSH
CSB-EP749891BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana 30S ribosomal protein S17 (rpsQ) CSB-YP749892BSH
CSB-EP749892BSH
CSB-BP749892BSH
CSB-MP749892BSH
CSB-EP749892BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana 50S ribosomal protein L18 (rplR) CSB-YP749893BSH
CSB-EP749893BSH
CSB-BP749893BSH
CSB-MP749893BSH
CSB-EP749893BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana 30S ribosomal protein S13 (rpsM) CSB-YP749894BSH
CSB-EP749894BSH
CSB-BP749894BSH
CSB-MP749894BSH
CSB-EP749894BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Aspartate--tRNA ligase (aspS), partial CSB-YP749895BSH
CSB-EP749895BSH
CSB-BP749895BSH
CSB-MP749895BSH
CSB-EP749895BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana 30S ribosomal protein S2 (rpsB) CSB-YP749896BSH
CSB-EP749896BSH
CSB-BP749896BSH
CSB-MP749896BSH
CSB-EP749896BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Uridylate kinase (pyrH) CSB-YP749897BSH
CSB-EP749897BSH
CSB-BP749897BSH
CSB-MP749897BSH
CSB-EP749897BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP749898BSH1
CSB-EP749898BSH1
CSB-BP749898BSH1
CSB-MP749898BSH1
CSB-EP749898BSH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Proline--tRNA ligase (proS) CSB-YP749899BSH
CSB-EP749899BSH
CSB-BP749899BSH
CSB-MP749899BSH
CSB-EP749899BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Putative membrane protein insertion efficiency factor (BQ05520) CSB-YP749900BSH
CSB-EP749900BSH
CSB-BP749900BSH
CSB-MP749900BSH
CSB-EP749900BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Transcriptional repressor NrdR (nrdR) CSB-YP749901BSH
CSB-EP749901BSH
CSB-BP749901BSH
CSB-MP749901BSH
CSB-EP749901BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP749902BSH
CSB-EP749902BSH
CSB-BP749902BSH
CSB-MP749902BSH
CSB-EP749902BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Guanylate kinase (gmk) CSB-YP749903BSH
CSB-EP749903BSH
CSB-BP749903BSH
CSB-MP749903BSH
CSB-EP749903BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Cysteine--tRNA ligase (cysS), partial CSB-YP749904BSH
CSB-EP749904BSH
CSB-BP749904BSH
CSB-MP749904BSH
CSB-EP749904BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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