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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria monocytogenes serotype 4b UPF0348 protein LMOf2365_2080 (LMOf2365_2080) CSB-YP751291LAAD
CSB-EP751291LAAD
CSB-BP751291LAAD
CSB-MP751291LAAD
CSB-EP751291LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Segregation and condensation protein A (scpA) CSB-YP751292LAAD
CSB-EP751292LAAD
CSB-BP751292LAAD
CSB-MP751292LAAD
CSB-EP751292LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Chorismate synthase (aroC) CSB-YP751293LAAD
CSB-EP751293LAAD
CSB-BP751293LAAD
CSB-MP751293LAAD
CSB-EP751293LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b CCA-adding enzyme (cca) CSB-YP751294LAAD
CSB-EP751294LAAD
CSB-BP751294LAAD
CSB-MP751294LAAD
CSB-EP751294LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Formate--tetrahydrofolate ligase (fhs), partial CSB-YP751295LAAD
CSB-EP751295LAAD
CSB-BP751295LAAD
CSB-MP751295LAAD
CSB-EP751295LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP751296LAAD
CSB-EP751296LAAD
CSB-BP751296LAAD
CSB-MP751296LAAD
CSB-EP751296LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Dihydroorotate dehydrogenase B (NAD (+)), electron transfer subunit (pyrK) CSB-YP751297LAAD
CSB-EP751297LAAD
CSB-BP751297LAAD
CSB-MP751297LAAD
CSB-EP751297LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Serine/threonine phosphatase stp (stp) CSB-YP751298LAAD
CSB-EP751298LAAD
CSB-BP751298LAAD
CSB-MP751298LAAD
CSB-EP751298LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 30S ribosomal protein S16 (rpsP) CSB-YP751299LAAD
CSB-EP751299LAAD
CSB-BP751299LAAD
CSB-MP751299LAAD
CSB-EP751299LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Ribosome maturation factor RimM (rimM) CSB-YP751300LAAD
CSB-EP751300LAAD
CSB-BP751300LAAD
CSB-MP751300LAAD
CSB-EP751300LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Translation initiation factor IF-3 (infC) CSB-YP751301LAAD
CSB-EP751301LAAD
CSB-BP751301LAAD
CSB-MP751301LAAD
CSB-EP751301LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP751302LAAD
CSB-EP751302LAAD
CSB-BP751302LAAD
CSB-MP751302LAAD
CSB-EP751302LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP751303LAAD
CSB-EP751303LAAD
CSB-BP751303LAAD
CSB-MP751303LAAD
CSB-EP751303LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative membrane protein insertion efficiency factor (LMOf2365_1694) CSB-YP751304LAAD
CSB-EP751304LAAD
CSB-BP751304LAAD
CSB-MP751304LAAD
CSB-EP751304LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0354 protein LMOf2365_1630 (LMOf2365_1630) CSB-YP751305LAAD
CSB-EP751305LAAD
CSB-BP751305LAAD
CSB-MP751305LAAD
CSB-EP751305LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Acetylglutamate kinase (argB) CSB-YP751306LAAD
CSB-EP751306LAAD
CSB-BP751306LAAD
CSB-MP751306LAAD
CSB-EP751306LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0173 metal-dependent hydrolase LMOf2365_1599 (LMOf2365_1599) CSB-YP751307LAAD
CSB-EP751307LAAD
CSB-BP751307LAAD
CSB-MP751307LAAD
CSB-EP751307LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP751308LAAD
CSB-EP751308LAAD
CSB-BP751308LAAD
CSB-MP751308LAAD
CSB-EP751308LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b L-lactate dehydrogenase 2 (ldh2) CSB-YP751309LAAD
CSB-EP751309LAAD
CSB-BP751309LAAD
CSB-MP751309LAAD
CSB-EP751309LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP751310LAAD
CSB-EP751310LAAD
CSB-BP751310LAAD
CSB-MP751310LAAD
CSB-EP751310LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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