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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bordetella bronchiseptica UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP749825BTY
CSB-EP749825BTY
CSB-BP749825BTY
CSB-MP749825BTY
CSB-EP749825BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Probable multidrug resistance protein norM (norM), partial CSB-YP749826BTY
CSB-EP749826BTY
CSB-BP749826BTY
CSB-MP749826BTY
CSB-EP749826BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica UvrABC system protein B (uvrB), partial CSB-YP749827BTY
CSB-EP749827BTY
CSB-BP749827BTY
CSB-MP749827BTY
CSB-EP749827BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Threonine--tRNA ligase (thrS), partial CSB-YP749828BTY
CSB-EP749828BTY
CSB-BP749828BTY
CSB-MP749828BTY
CSB-EP749828BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Porphobilinogen deaminase (hemC) CSB-YP749829BTY
CSB-EP749829BTY
CSB-BP749829BTY
CSB-MP749829BTY
CSB-EP749829BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 50S ribosomal protein L35 (rpmI) CSB-YP749830BTY
CSB-EP749830BTY
CSB-BP749830BTY
CSB-MP749830BTY
CSB-EP749830BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Acetylornithine aminotransferase 1 (argD1) CSB-YP749831BTY
CSB-EP749831BTY
CSB-BP749831BTY
CSB-MP749831BTY
CSB-EP749831BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 30S ribosomal protein S18 (rpsR) CSB-YP749832BTY
CSB-EP749832BTY
CSB-BP749832BTY
CSB-MP749832BTY
CSB-EP749832BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Protease HtpX homolog (htpX), partial CSB-YP749833BTY1
CSB-EP749833BTY1
CSB-BP749833BTY1
CSB-MP749833BTY1
CSB-EP749833BTY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica NAD-dependent protein deacetylase (cobB) CSB-YP749834BTY
CSB-EP749834BTY
CSB-BP749834BTY
CSB-MP749834BTY
CSB-EP749834BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Putative anti-sigma factor antagonist BtrV (btrV) CSB-YP749835BTY
CSB-EP749835BTY
CSB-BP749835BTY
CSB-MP749835BTY
CSB-EP749835BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Transaldolase (tal) CSB-YP749836BTY
CSB-EP749836BTY
CSB-BP749836BTY
CSB-MP749836BTY
CSB-EP749836BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP749837BTY
CSB-EP749837BTY
CSB-BP749837BTY
CSB-MP749837BTY
CSB-EP749837BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Phosphonates import ATP-binding protein PhnC (phnC) CSB-YP749838BTY
CSB-EP749838BTY
CSB-BP749838BTY
CSB-MP749838BTY
CSB-EP749838BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Glucose-6-phosphate isomerase (pgi), partial CSB-YP749839BTY
CSB-EP749839BTY
CSB-BP749839BTY
CSB-MP749839BTY
CSB-EP749839BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Ornithine aminotransferase (rocD) CSB-YP749840BTY
CSB-EP749840BTY
CSB-BP749840BTY
CSB-MP749840BTY
CSB-EP749840BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Virginiamycin B lyase (vgb) CSB-YP749841BTY
CSB-EP749841BTY
CSB-BP749841BTY
CSB-MP749841BTY
CSB-EP749841BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Glutamate 5-kinase (proB) CSB-YP749842BTY
CSB-EP749842BTY
CSB-BP749842BTY
CSB-MP749842BTY
CSB-EP749842BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Methionyl-tRNA formyltransferase (fmt) CSB-YP749843BTY
CSB-EP749843BTY
CSB-BP749843BTY
CSB-MP749843BTY
CSB-EP749843BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Lipoyl synthase (lipA) CSB-YP749844BTY
CSB-EP749844BTY
CSB-BP749844BTY
CSB-MP749844BTY
CSB-EP749844BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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