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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Corynebacterium glutamicum Urease accessory protein UreF (ureF) CSB-YP751911DWY
CSB-EP751911DWY
CSB-BP751911DWY
CSB-MP751911DWY
CSB-EP751911DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus O-acetyltransferase OatA (oatA), partial CSB-YP751912SUL
CSB-EP751912SUL
CSB-BP751912SUL
CSB-MP751912SUL
CSB-EP751912SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Oxygen sensor histidine kinase nreB (nreB) CSB-YP751913SUL
CSB-EP751913SUL
CSB-BP751913SUL
CSB-MP751913SUL
CSB-EP751913SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator sarV (sarV) CSB-YP751914SUL
CSB-EP751914SUL
CSB-BP751914SUL
CSB-MP751914SUL
CSB-EP751914SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L5 (rplE) CSB-YP751915SUL
CSB-EP751915SUL
CSB-BP751915SUL
CSB-MP751915SUL
CSB-EP751915SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0457 protein MW2099 (MW2099) CSB-YP751916SUL
CSB-EP751916SUL
CSB-BP751916SUL
CSB-MP751916SUL
CSB-EP751916SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase gamma chain (atpG) CSB-YP751917SUL
CSB-EP751917SUL
CSB-BP751917SUL
CSB-MP751917SUL
CSB-EP751917SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Diacylglycerol kinase (dagK) CSB-YP751918SUL
CSB-EP751918SUL
CSB-BP751918SUL
CSB-MP751918SUL
CSB-EP751918SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0342 protein MW1786 (MW1786) CSB-YP751919SUL
CSB-EP751919SUL
CSB-BP751919SUL
CSB-MP751919SUL
CSB-EP751919SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTPase obg (obg) CSB-YP751920SUL
CSB-EP751920SUL
CSB-BP751920SUL
CSB-MP751920SUL
CSB-EP751920SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S20 (rpsT) CSB-YP751921SUL
CSB-EP751921SUL
CSB-BP751921SUL
CSB-MP751921SUL
CSB-EP751921SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative branched-chain amino acid carrier protein MW1297 (MW1297), partial CSB-YP751922SUL
CSB-EP751922SUL
CSB-BP751922SUL
CSB-MP751922SUL
CSB-EP751922SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein GlcT (glcT) CSB-YP751923SUL
CSB-EP751923SUL
CSB-BP751923SUL
CSB-MP751923SUL
CSB-EP751923SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease J 2 (MW1158), partial CSB-YP751924SUL
CSB-EP751924SUL
CSB-BP751924SUL
CSB-MP751924SUL
CSB-EP751924SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Carbamate kinase 1 (arcC1) CSB-YP751925SUL
CSB-EP751925SUL
CSB-BP751925SUL
CSB-MP751925SUL
CSB-EP751925SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP751926SUL
CSB-EP751926SUL
CSB-BP751926SUL
CSB-MP751926SUL
CSB-EP751926SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0344 protein MW0851 (MW0851), partial CSB-YP751927SUL1
CSB-EP751927SUL1
CSB-BP751927SUL1
CSB-MP751927SUL1
CSB-EP751927SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative sporulation transcription regulator WhiA (whiA) CSB-YP751928SUL
CSB-EP751928SUL
CSB-BP751928SUL
CSB-MP751928SUL
CSB-EP751928SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Response regulator saeR (saeR) CSB-YP751929SUL
CSB-EP751929SUL
CSB-BP751929SUL
CSB-MP751929SUL
CSB-EP751929SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative antiporter subunit mnhB2 (mnhB2), partial CSB-YP751930SUL1
CSB-EP751930SUL1
CSB-BP751930SUL1
CSB-MP751930SUL1
CSB-EP751930SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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