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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neurospora crassa tRNA-dihydrouridine (47) synthase [NAD (P) (+)] (dus-3), partial CSB-YP752574NHA
CSB-EP752574NHA
CSB-BP752574NHA
CSB-MP752574NHA
CSB-EP752574NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mediator of RNA polymerase II transcription subunit 12 (srb-8), partial CSB-YP752575NHA
CSB-EP752575NHA
CSB-BP752575NHA
CSB-MP752575NHA
CSB-EP752575NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Sorting nexin-4 (snx-4) CSB-YP752576NHA
CSB-EP752576NHA
CSB-BP752576NHA
CSB-MP752576NHA
CSB-EP752576NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Pre-mRNA-processing ATP-dependent RNA helicase prp-5 (prp-5), partial CSB-YP752577NHA
CSB-EP752577NHA
CSB-BP752577NHA
CSB-MP752577NHA
CSB-EP752577NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein pxr-1 (pxr-1) CSB-YP752578NHA
CSB-EP752578NHA
CSB-BP752578NHA
CSB-MP752578NHA
CSB-EP752578NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa ATP synthase subunit d, mitochondrial (atp-7) CSB-YP752579NHA
CSB-EP752579NHA
CSB-BP752579NHA
CSB-MP752579NHA
CSB-EP752579NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Bifunctional glutamate/proline--tRNA ligase (EPRS) CSB-YP752580DXU
CSB-EP752580DXU
CSB-BP752580DXU
CSB-MP752580DXU
CSB-EP752580DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dryopteris crassirhizoma Plastocyanin (PETE) CSB-YP752581DMT
CSB-EP752581DMT
CSB-BP752581DMT
CSB-MP752581DMT
CSB-EP752581DMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Transketolase, chloroplastic, partial CSB-YP752582ZAX
CSB-EP752582ZAX
CSB-BP752582ZAX
CSB-MP752582ZAX
CSB-EP752582ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arsenite oxidase subunit AioA (aioA), partial CSB-YP752583AGN
CSB-EP752583AGN
CSB-BP752583AGN
CSB-MP752583AGN
CSB-EP752583AGN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Crossover junction endonuclease MUS81 (mus81), partial CSB-YP752584DIL
CSB-EP752584DIL
CSB-BP752584DIL
CSB-MP752584DIL
CSB-EP752584DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio SUMO-activating enzyme subunit 2 (uba2), partial CSB-YP752585DIL
CSB-EP752585DIL
CSB-BP752585DIL
CSB-MP752585DIL
CSB-EP752585DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cytoplasmic polyadenylation element-binding protein 4 (cpeb4), partial CSB-YP752586DIL
CSB-EP752586DIL
CSB-BP752586DIL
CSB-MP752586DIL
CSB-EP752586DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Calumenin-B (calub) CSB-YP752587DIL
CSB-EP752587DIL
CSB-BP752587DIL
CSB-MP752587DIL
CSB-EP752587DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Probable RNA-binding protein EIF1AD (eif1ad) CSB-YP752588DIL
CSB-EP752588DIL
CSB-BP752588DIL
CSB-MP752588DIL
CSB-EP752588DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Sugar phosphate exchanger 2 (slc37a2), partial CSB-YP752589DIL
CSB-EP752589DIL
CSB-BP752589DIL
CSB-MP752589DIL
CSB-EP752589DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Neurocalcin-delta (ncald) CSB-YP752590XBE
CSB-EP752590XBE
CSB-BP752590XBE
CSB-MP752590XBE
CSB-EP752590XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio ADP-ribosylation factor-like protein 2-binding protein (arl2bp) CSB-YP752591DIL
CSB-EP752591DIL
CSB-BP752591DIL
CSB-MP752591DIL
CSB-EP752591DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Histamine N-methyltransferase B (hnmt-b) CSB-YP752592XBE
CSB-EP752592XBE
CSB-BP752592XBE
CSB-MP752592XBE
CSB-EP752592XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis DNA-binding protein inhibitor ID-3-B (id3-b) CSB-YP752593XBE
CSB-EP752593XBE
CSB-BP752593XBE
CSB-MP752593XBE
CSB-EP752593XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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