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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis Serine/threonine-protein phosphatase 1 regulatory subunit 10 (ppp1r10), partial CSB-YP753556XBE
CSB-EP753556XBE
CSB-BP753556XBE
CSB-MP753556XBE
CSB-EP753556XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Transmembrane protein 56-A (tmem56-a), partial CSB-YP753557XBE1
CSB-EP753557XBE1
CSB-BP753557XBE1
CSB-MP753557XBE1
CSB-EP753557XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein asunder homolog (asun), partial CSB-YP753558XBE
CSB-EP753558XBE
CSB-BP753558XBE
CSB-MP753558XBE
CSB-EP753558XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0694 transmembrane protein C14orf109 homolog B, partial CSB-YP753559XBE1
CSB-EP753559XBE1
CSB-BP753559XBE1
CSB-MP753559XBE1
CSB-EP753559XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Promethin-B (tmem159-b), partial CSB-YP753560XBE1
CSB-EP753560XBE1
CSB-BP753560XBE1
CSB-MP753560XBE1
CSB-EP753560XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Secretory carrier-associated membrane protein 5B (scamp5-b), partial CSB-YP753561XBE1
CSB-EP753561XBE1
CSB-BP753561XBE1
CSB-MP753561XBE1
CSB-EP753561XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Leucine-rich repeat and WD repeat-containing protein 1 (lrwd1), partial CSB-YP753562XBE
CSB-EP753562XBE
CSB-BP753562XBE
CSB-MP753562XBE
CSB-EP753562XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0515 protein C19orf66 homolog CSB-YP753563XBE
CSB-EP753563XBE
CSB-BP753563XBE
CSB-MP753563XBE
CSB-EP753563XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Inosine-5'-monophosphate dehydrogenase 1a (impdh1a), partial CSB-YP753564DIL
CSB-EP753564DIL
CSB-BP753564DIL
CSB-MP753564DIL
CSB-EP753564DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Tetraspanin-9 (tspan9), partial CSB-YP753565DIL1
CSB-EP753565DIL1
CSB-BP753565DIL1
CSB-MP753565DIL1
CSB-EP753565DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative peptidyl-tRNA hydrolase PTRHD1 (PTRHD1) CSB-YP753566HU
CSB-EP753566HU
CSB-BP753566HU
CSB-MP753566HU
CSB-EP753566HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 3-hydroxyacyl-CoA dehydratase (ptplad2), partial CSB-YP753567XBE1
CSB-EP753567XBE1
CSB-BP753567XBE1
CSB-MP753567XBE1
CSB-EP753567XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis PRKR-interacting protein 1 homolog (prkrip1) CSB-YP753568XBE
CSB-EP753568XBE
CSB-BP753568XBE
CSB-MP753568XBE
CSB-EP753568XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nurim (nrm), partial CSB-YP753569XBE1
CSB-EP753569XBE1
CSB-BP753569XBE1
CSB-MP753569XBE1
CSB-EP753569XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 60S ribosomal export protein NMD3 (nmd3), partial CSB-YP753570XBE
CSB-EP753570XBE
CSB-BP753570XBE
CSB-MP753570XBE
CSB-EP753570XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Leucine-rich repeat flightless-interacting protein 2 (lrrfip2), partial CSB-YP753571XBE
CSB-EP753571XBE
CSB-BP753571XBE
CSB-MP753571XBE
CSB-EP753571XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Histone-lysine N-methyltransferase SUV420H1-A (suv420h1-a), partial CSB-YP753572XBE
CSB-EP753572XBE
CSB-BP753572XBE
CSB-MP753572XBE
CSB-EP753572XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis N-alpha-acetyltransferase 50 (naa50) CSB-YP753573XBE
CSB-EP753573XBE
CSB-BP753573XBE
CSB-MP753573XBE
CSB-EP753573XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Selenoprotein M (sepm) CSB-YP753574XBE
CSB-EP753574XBE
CSB-BP753574XBE
CSB-MP753574XBE
CSB-EP753574XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Probable alpha-ketoglutarate-dependent dioxygenase ABH5 (alkbh5) CSB-YP753575XBE
CSB-EP753575XBE
CSB-BP753575XBE
CSB-MP753575XBE
CSB-EP753575XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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