Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Protochlamydia amoebophila 50S ribosomal protein L5 (rplE) CSB-YP753957PAAD
CSB-EP753957PAAD
CSB-BP753957PAAD
CSB-MP753957PAAD
CSB-EP753957PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 30S ribosomal protein S3 (rpsC) CSB-YP753958PAAD
CSB-EP753958PAAD
CSB-BP753958PAAD
CSB-MP753958PAAD
CSB-EP753958PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP753959PAAD
CSB-EP753959PAAD
CSB-BP753959PAAD
CSB-MP753959PAAD
CSB-EP753959PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Nucleoside diphosphate kinase 2 (ndk2) CSB-YP753960PAAD
CSB-EP753960PAAD
CSB-BP753960PAAD
CSB-MP753960PAAD
CSB-EP753960PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP753961PAAD
CSB-EP753961PAAD
CSB-BP753961PAAD
CSB-MP753961PAAD
CSB-EP753961PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila tRNA pseudouridine synthase A 1 (truA1) CSB-YP753962PAAD
CSB-EP753962PAAD
CSB-BP753962PAAD
CSB-MP753962PAAD
CSB-EP753962PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila Ribonuclease 3 (rnc) CSB-YP753963PAAD
CSB-EP753963PAAD
CSB-BP753963PAAD
CSB-MP753963PAAD
CSB-EP753963PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP753964PAAD
CSB-EP753964PAAD
CSB-BP753964PAAD
CSB-MP753964PAAD
CSB-EP753964PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protochlamydia amoebophila 3-dehydroquinate synthase (aroB) CSB-YP753965PAAD
CSB-EP753965PAAD
CSB-BP753965PAAD
CSB-MP753965PAAD
CSB-EP753965PAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Proline-rich protein 3 (Prr3) CSB-YP753966RA
CSB-EP753966RA
CSB-BP753966RA
CSB-MP753966RA
CSB-EP753966RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Uncharacterized protein C6orf47 homolog (G4) CSB-YP753967RA
CSB-EP753967RA
CSB-BP753967RA
CSB-MP753967RA
CSB-EP753967RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat MutS protein homolog 5 (Msh5), partial CSB-YP753968RA
CSB-EP753968RA
CSB-BP753968RA
CSB-MP753968RA
CSB-EP753968RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Estradiol 17-beta-dehydrogenase 8 (Hsd17b8) CSB-YP753969RA
CSB-EP753969RA
CSB-BP753969RA
CSB-MP753969RA
CSB-EP753969RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus ATP synthase subunit b (atpF), partial CSB-YP753970BFP
CSB-EP753970BFP
CSB-BP753970BFP
CSB-MP753970BFP
CSB-EP753970BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus ATP synthase gamma chain (atpG) CSB-YP753971BFP
CSB-EP753971BFP
CSB-BP753971BFP
CSB-MP753971BFP
CSB-EP753971BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP753972BFP
CSB-EP753972BFP
CSB-BP753972BFP
CSB-MP753972BFP
CSB-EP753972BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus GTPase obg (obg) CSB-YP753973BFP
CSB-EP753973BFP
CSB-BP753973BFP
CSB-MP753973BFP
CSB-EP753973BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Trigger factor (tig) CSB-YP753974BFP
CSB-EP753974BFP
CSB-BP753974BFP
CSB-MP753974BFP
CSB-EP753974BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP753975BFP
CSB-EP753975BFP
CSB-BP753975BFP
CSB-MP753975BFP
CSB-EP753975BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bdellovibrio bacteriovorus Bifunctional protein GlmU (glmU) CSB-YP753976BFP
CSB-EP753976BFP
CSB-BP753976BFP
CSB-MP753976BFP
CSB-EP753976BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>