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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterobacter sp. Glycogen debranching enzyme (glgX), partial CSB-YP001446EIZ
CSB-EP001446EIZ
CSB-BP001446EIZ
CSB-MP001446EIZ
CSB-EP001446EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. ATP synthase subunit a (atpB), partial CSB-YP015070EIZ1
CSB-EP015070EIZ1
CSB-BP015070EIZ1
CSB-MP015070EIZ1
CSB-EP015070EIZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter sp. ATP synthase subunit alpha (atpA), partial CSB-YP002344EIZ
CSB-EP002344EIZ
CSB-BP002344EIZ
CSB-MP002344EIZ
CSB-EP002344EIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum tRNA pseudouridine synthase A (truA) CSB-YP019086PZU
CSB-EP019086PZU
CSB-BP019086PZU
CSB-MP019086PZU
CSB-EP019086PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum arsenaticum Protein pelota homolog (pelA) CSB-YP017778PZU
CSB-EP017778PZU
CSB-BP017778PZU
CSB-MP017778PZU
CSB-EP017778PZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Malate dehydrogenase (mdh) CSB-YP013623RLG
CSB-EP013623RLG
CSB-BP013623RLG
CSB-MP013623RLG
CSB-EP013623RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides ATP synthase subunit delta (atpH) CSB-YP002355RLG
CSB-EP002355RLG
CSB-BP002355RLG
CSB-MP002355RLG
CSB-EP002355RLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salinispora tropica Phosphoenolpyruvate carboxykinase [GTP] (pckG), partial CSB-YP017614STD
CSB-EP017614STD
CSB-BP017614STD
CSB-MP017614STD
CSB-EP017614STD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Peptide chain release factor 1 (prfA) CSB-YP015197CVX
CSB-EP015197CVX
CSB-BP015197CVX
CSB-MP015197CVX
CSB-EP015197CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Porphobilinogen deaminase (hemC) CSB-YP010524CVX
CSB-EP010524CVX
CSB-BP010524CVX
CSB-MP010524CVX
CSB-EP010524CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus GTPase Era (era) CSB-YP007759CVX
CSB-EP007759CVX
CSB-BP007759CVX
CSB-MP007759CVX
CSB-EP007759CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Methionyl-tRNA formyltransferase (fmt) CSB-YP015150CVX
CSB-EP015150CVX
CSB-BP015150CVX
CSB-MP015150CVX
CSB-EP015150CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caldicellulosiruptor saccharolyticus Elongation factor Ts (tsf) CSB-YP025124CVX
CSB-EP025124CVX
CSB-BP025124CVX
CSB-MP025124CVX
CSB-EP025124CVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 3-ketoacyl-CoA thiolase (fadI), partial CSB-YP001117YAL
CSB-EP001117YAL
CSB-BP001117YAL
CSB-MP001117YAL
CSB-EP001117YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582YAL
CSB-EP009582YAL
CSB-BP009582YAL
CSB-MP009582YAL
CSB-EP009582YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033YAL
CSB-EP018033YAL
CSB-BP018033YAL
CSB-MP018033YAL
CSB-EP018033YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis 6-phosphofructokinase (pfkA) CSB-YP017822YAL
CSB-EP017822YAL
CSB-BP017822YAL
CSB-MP017822YAL
CSB-EP017822YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis ATP synthase subunit alpha (atpA), partial CSB-YP002344YAL
CSB-EP002344YAL
CSB-BP002344YAL
CSB-MP002344YAL
CSB-EP002344YAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Elongation factor G (fusA), partial CSB-YP009373PZP
CSB-EP009373PZP
CSB-BP009373PZP
CSB-MP009373PZP
CSB-EP009373PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917PZP
CSB-EP022917PZP
CSB-BP022917PZP
CSB-MP022917PZP
CSB-EP022917PZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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