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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii 60S ribosomal protein L7 (RPL7) CSB-YP755119DOT
CSB-EP755119DOT
CSB-BP755119DOT
CSB-MP755119DOT
CSB-EP755119DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Small COPII coat GTPase SAR1 (SAR1) CSB-YP755120DOT
CSB-EP755120DOT
CSB-BP755120DOT
CSB-MP755120DOT
CSB-EP755120DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Rhomboid protein 2 (RBD2), partial CSB-YP755121DOT1
CSB-EP755121DOT1
CSB-BP755121DOT1
CSB-MP755121DOT1
CSB-EP755121DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ribulose-phosphate 3-epimerase (RPE1) CSB-YP755122DOT
CSB-EP755122DOT
CSB-BP755122DOT
CSB-MP755122DOT
CSB-EP755122DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Inheritance of peroxisomes protein 1 (INP1) CSB-YP755123DOT
CSB-EP755123DOT
CSB-BP755123DOT
CSB-MP755123DOT
CSB-EP755123DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii U3 small nucleolar ribonucleoprotein protein IMP4 (IMP4) CSB-YP755124DOT
CSB-EP755124DOT
CSB-BP755124DOT
CSB-MP755124DOT
CSB-EP755124DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii FAS1 domain-containing protein AER383W (AER383W) CSB-YP755125DOT
CSB-EP755125DOT
CSB-BP755125DOT
CSB-MP755125DOT
CSB-EP755125DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-mRNA-splicing factor CEF1 (CEF1) CSB-YP755126DOT
CSB-EP755126DOT
CSB-BP755126DOT
CSB-MP755126DOT
CSB-EP755126DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DNA repair and recombination protein RAD52 (RAD52) CSB-YP755127DOT
CSB-EP755127DOT
CSB-BP755127DOT
CSB-MP755127DOT
CSB-EP755127DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Histone acetyltransferase GCN5 (GCN5) CSB-YP755128DOT
CSB-EP755128DOT
CSB-BP755128DOT
CSB-MP755128DOT
CSB-EP755128DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Spindle pole body component 110 (SPC110), partial CSB-YP755129DOT
CSB-EP755129DOT
CSB-BP755129DOT
CSB-MP755129DOT
CSB-EP755129DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii FK506-binding protein 3 (FPR3) CSB-YP755130DOT
CSB-EP755130DOT
CSB-BP755130DOT
CSB-MP755130DOT
CSB-EP755130DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Damage-regulated import facilitator 1 (DIF1) CSB-YP755131DOT
CSB-EP755131DOT
CSB-BP755131DOT
CSB-MP755131DOT
CSB-EP755131DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Biogenesis of lysosome-related organelles complex 1 subunit BLS1 (BLS1) CSB-YP755132DOT
CSB-EP755132DOT
CSB-BP755132DOT
CSB-MP755132DOT
CSB-EP755132DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Arrestin-related trafficking adapter 10 (ART10) CSB-YP755133DOT
CSB-EP755133DOT
CSB-BP755133DOT
CSB-MP755133DOT
CSB-EP755133DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent RNA helicase MAK5 (MAK5), partial CSB-YP755134DOT
CSB-EP755134DOT
CSB-BP755134DOT
CSB-MP755134DOT
CSB-EP755134DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mediator of RNA polymerase II transcription subunit 2 (MED2) CSB-YP755135DOT
CSB-EP755135DOT
CSB-BP755135DOT
CSB-MP755135DOT
CSB-EP755135DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 1,4-alpha-glucan-branching enzyme (GLC3), partial CSB-YP755136DOT
CSB-EP755136DOT
CSB-BP755136DOT
CSB-MP755136DOT
CSB-EP755136DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Probable trans-2-enoyl-CoA reductase, mitochondrial (ETR1) CSB-YP755137DOT
CSB-EP755137DOT
CSB-BP755137DOT
CSB-MP755137DOT
CSB-EP755137DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii UPF0508 protein AEL141W (AEL141W), partial CSB-YP755138DOT
CSB-EP755138DOT
CSB-BP755138DOT
CSB-MP755138DOT
CSB-EP755138DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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