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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Cyclin-P3-1 (CYCP3-1) CSB-YP755199OFG
CSB-EP755199OFG
CSB-BP755199OFG
CSB-MP755199OFG
CSB-EP755199OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Secretory carrier-associated membrane protein 4 (SCAMP4), partial CSB-YP755200OFG1
CSB-EP755200OFG1
CSB-BP755200OFG1
CSB-MP755200OFG1
CSB-EP755200OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0271698 (DDB_G0271698) CSB-YP755201DKK
CSB-EP755201DKK
CSB-BP755201DKK
CSB-MP755201DKK
CSB-EP755201DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum SH3 and FCH domain-containing protein DDB_G0271676 (DDB_G0271676), partial CSB-YP755202DKK
CSB-EP755202DKK
CSB-BP755202DKK
CSB-MP755202DKK
CSB-EP755202DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cell division cycle protein 123 homolog (cdc123) CSB-YP755203DKK
CSB-EP755203DKK
CSB-BP755203DKK
CSB-MP755203DKK
CSB-EP755203DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ribose-phosphate pyrophosphokinase B (prsB) CSB-YP755204DKK
CSB-EP755204DKK
CSB-BP755204DKK
CSB-MP755204DKK
CSB-EP755204DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum UPF0512 protein B (DDB_G0272228) CSB-YP755205DKK
CSB-EP755205DKK
CSB-BP755205DKK
CSB-MP755205DKK
CSB-EP755205DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Uncharacterized protein DDB_G0272188 (DDB_G0272188) CSB-YP755206DKK
CSB-EP755206DKK
CSB-BP755206DKK
CSB-MP755206DKK
CSB-EP755206DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Calcium-binding protein NCSA (ncsA) CSB-YP755207DKK
CSB-EP755207DKK
CSB-BP755207DKK
CSB-MP755207DKK
CSB-EP755207DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Calcineurin B-like protein 4 (CBL4) CSB-YP755208OFG
CSB-EP755208OFG
CSB-BP755208OFG
CSB-MP755208OFG
CSB-EP755208OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Flap endonuclease 1-B (FEN-1b) CSB-YP755209OFG
CSB-EP755209OFG
CSB-BP755209OFG
CSB-MP755209OFG
CSB-EP755209OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human E3 ubiquitin-protein ligase Hakai (CBLL1) CSB-YP755210HU
CSB-EP755210HU
CSB-BP755210HU
CSB-MP755210HU
CSB-EP755210HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse ATP-dependent DNA helicase Q4 (Recql4), partial CSB-YP755211MO
CSB-EP755211MO
CSB-BP755211MO
CSB-MP755211MO
CSB-EP755211MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glomerella lagenarium Mitogen-activated protein kinase HOG1 (HOG1) CSB-YP755212GCM
CSB-EP755212GCM
CSB-BP755212GCM
CSB-MP755212GCM
CSB-EP755212GCM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio anguillarum Na (+)-translocating NADH-quinone reductase subunit C (nqrC), partial CSB-YP755213VEV1
CSB-EP755213VEV1
CSB-BP755213VEV1
CSB-MP755213VEV1
CSB-EP755213VEV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus candidus Phospholipase A2, beta bungarotoxin A4 chain CSB-YP755214BPN
CSB-EP755214BPN
CSB-BP755214BPN
CSB-MP755214BPN
CSB-EP755214BPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus kaustophilus Bifunctional protein FolD (folD) CSB-YP755215GAAA
CSB-EP755215GAAA
CSB-BP755215GAAA
CSB-MP755215GAAA
CSB-EP755215GAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant F420-0:gamma-glutamyl ligase (cofE) CSB-YP755216FNW
CSB-EP755216FNW
CSB-BP755216FNW
CSB-MP755216FNW
CSB-EP755216FNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Calcitonin receptor-stimulating peptide 1 (CRSP1) CSB-YP755217BO
CSB-EP755217BO
CSB-BP755217BO
CSB-MP755217BO
CSB-EP755217BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Translationally-controlled tumor protein homolog (Tctp) CSB-YP755218BTT
CSB-EP755218BTT
CSB-BP755218BTT
CSB-MP755218BTT
CSB-EP755218BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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