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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter sulfurreducens Recombination protein RecR (recR) CSB-YP755039GBK
CSB-EP755039GBK
CSB-BP755039GBK
CSB-MP755039GBK
CSB-EP755039GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens DNA replication and repair protein recF (recF) CSB-YP755040GBK
CSB-EP755040GBK
CSB-BP755040GBK
CSB-MP755040GBK
CSB-EP755040GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Pyrrolidone-carboxylate peptidase (pcp) CSB-YP755041LAW
CSB-EP755041LAW
CSB-BP755041LAW
CSB-MP755041LAW
CSB-EP755041LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP755042LAW
CSB-EP755042LAW
CSB-BP755042LAW
CSB-MP755042LAW
CSB-EP755042LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP755043LAW
CSB-EP755043LAW
CSB-BP755043LAW
CSB-MP755043LAW
CSB-EP755043LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii 50S ribosomal protein L20 (rplT) CSB-YP755044LAW
CSB-EP755044LAW
CSB-BP755044LAW
CSB-MP755044LAW
CSB-EP755044LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Uncharacterized RNA methyltransferase LJ_1606 (LJ_1606) CSB-YP755045LAW
CSB-EP755045LAW
CSB-BP755045LAW
CSB-MP755045LAW
CSB-EP755045LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP755046LAW
CSB-EP755046LAW
CSB-BP755046LAW
CSB-MP755046LAW
CSB-EP755046LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Phosphate acyltransferase (plsX) CSB-YP755047LAW
CSB-EP755047LAW
CSB-BP755047LAW
CSB-MP755047LAW
CSB-EP755047LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii 50S ribosomal protein L19 (rplS) CSB-YP755048LAW
CSB-EP755048LAW
CSB-BP755048LAW
CSB-MP755048LAW
CSB-EP755048LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Heat-inducible transcription repressor HrcA (hrcA) CSB-YP755049LAW
CSB-EP755049LAW
CSB-BP755049LAW
CSB-MP755049LAW
CSB-EP755049LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Enolase 3 (eno3) CSB-YP755050LAW
CSB-EP755050LAW
CSB-BP755050LAW
CSB-MP755050LAW
CSB-EP755050LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Putative phosphotransferase LJ_1326 (LJ_1326) CSB-YP755051LAW
CSB-EP755051LAW
CSB-BP755051LAW
CSB-MP755051LAW
CSB-EP755051LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Aspartate carbamoyltransferase (pyrB) CSB-YP755052LAW
CSB-EP755052LAW
CSB-BP755052LAW
CSB-MP755052LAW
CSB-EP755052LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP755053LAW
CSB-EP755053LAW
CSB-BP755053LAW
CSB-MP755053LAW
CSB-EP755053LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Cytidylate kinase (cmk) CSB-YP755054LAW
CSB-EP755054LAW
CSB-BP755054LAW
CSB-MP755054LAW
CSB-EP755054LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii 50S ribosomal protein L32 (rpmF) CSB-YP755055LAW
CSB-EP755055LAW
CSB-BP755055LAW
CSB-MP755055LAW
CSB-EP755055LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii 30S ribosomal protein S15 (rpsO) CSB-YP755056LAW
CSB-EP755056LAW
CSB-BP755056LAW
CSB-MP755056LAW
CSB-EP755056LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Isoleucine--tRNA ligase (ileS), partial CSB-YP755057LAW
CSB-EP755057LAW
CSB-BP755057LAW
CSB-MP755057LAW
CSB-EP755057LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Phosphoglucosamine mutase (glmM) CSB-YP755058LAW
CSB-EP755058LAW
CSB-BP755058LAW
CSB-MP755058LAW
CSB-EP755058LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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