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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio vulnificus 30S ribosomal protein S16 (rpsP) CSB-YP755582VCQ
CSB-EP755582VCQ
CSB-BP755582VCQ
CSB-MP755582VCQ
CSB-EP755582VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Sugar fermentation stimulation protein homolog (sfsA) CSB-YP755583VCQ
CSB-EP755583VCQ
CSB-BP755583VCQ
CSB-MP755583VCQ
CSB-EP755583VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phosphoglucosamine mutase (glmM) CSB-YP755584VCQ
CSB-EP755584VCQ
CSB-BP755584VCQ
CSB-MP755584VCQ
CSB-EP755584VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP755585VCQ
CSB-EP755585VCQ
CSB-BP755585VCQ
CSB-MP755585VCQ
CSB-EP755585VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Proline--tRNA ligase (proS), partial CSB-YP755586VCQ
CSB-EP755586VCQ
CSB-BP755586VCQ
CSB-MP755586VCQ
CSB-EP755586VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus [Protein-PII] uridylyltransferase (glnD), partial CSB-YP755587VCQ
CSB-EP755587VCQ
CSB-BP755587VCQ
CSB-MP755587VCQ
CSB-EP755587VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP755588VCQ
CSB-EP755588VCQ
CSB-BP755588VCQ
CSB-MP755588VCQ
CSB-EP755588VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP755589VCQ
CSB-EP755589VCQ
CSB-BP755589VCQ
CSB-MP755589VCQ
CSB-EP755589VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP755590VCQ
CSB-EP755590VCQ
CSB-BP755590VCQ
CSB-MP755590VCQ
CSB-EP755590VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP755591VCQ
CSB-EP755591VCQ
CSB-BP755591VCQ
CSB-MP755591VCQ
CSB-EP755591VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP755592VCQ
CSB-EP755592VCQ
CSB-BP755592VCQ
CSB-MP755592VCQ
CSB-EP755592VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP755593VCQ
CSB-EP755593VCQ
CSB-BP755593VCQ
CSB-MP755593VCQ
CSB-EP755593VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus tRNA (mo5U34)-methyltransferase (cmoB) CSB-YP755594VCQ
CSB-EP755594VCQ
CSB-BP755594VCQ
CSB-MP755594VCQ
CSB-EP755594VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus L-fucose isomerase (fucI), partial CSB-YP755595VCQ
CSB-EP755595VCQ
CSB-BP755595VCQ
CSB-MP755595VCQ
CSB-EP755595VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Disulfide bond formation protein B (dsbB), partial CSB-YP755596VCQ1
CSB-EP755596VCQ1
CSB-BP755596VCQ1
CSB-MP755596VCQ1
CSB-EP755596VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP755597VCQ
CSB-EP755597VCQ
CSB-BP755597VCQ
CSB-MP755597VCQ
CSB-EP755597VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Urocanate hydratase (hutU), partial CSB-YP755598VCQ
CSB-EP755598VCQ
CSB-BP755598VCQ
CSB-MP755598VCQ
CSB-EP755598VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Molybdenum cofactor guanylyltransferase (mobA) CSB-YP755599VCQ
CSB-EP755599VCQ
CSB-BP755599VCQ
CSB-MP755599VCQ
CSB-EP755599VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein ProQ homolog (proQ) CSB-YP755600VCQ
CSB-EP755600VCQ
CSB-BP755600VCQ
CSB-MP755600VCQ
CSB-EP755600VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Catalase-peroxidase (katG), partial CSB-YP755601VCQ
CSB-EP755601VCQ
CSB-BP755601VCQ
CSB-MP755601VCQ
CSB-EP755601VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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