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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 50S ribosomal protein L1 (rplA) CSB-YP755542WBQ
CSB-EP755542WBQ
CSB-BP755542WBQ
CSB-MP755542WBQ
CSB-EP755542WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP755543WBQ
CSB-EP755543WBQ
CSB-BP755543WBQ
CSB-MP755543WBQ
CSB-EP755543WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan--tRNA ligase (trpS) CSB-YP755544WBQ
CSB-EP755544WBQ
CSB-BP755544WBQ
CSB-MP755544WBQ
CSB-EP755544WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP755545WBQ
CSB-EP755545WBQ
CSB-BP755545WBQ
CSB-MP755545WBQ
CSB-EP755545WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine--tRNA ligase (argS), partial CSB-YP755546WBQ
CSB-EP755546WBQ
CSB-BP755546WBQ
CSB-MP755546WBQ
CSB-EP755546WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine biosynthesis bifunctional protein HisIE (hisI) CSB-YP755547WBQ
CSB-EP755547WBQ
CSB-BP755547WBQ
CSB-MP755547WBQ
CSB-EP755547WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP755548WBQ1
CSB-EP755548WBQ1
CSB-BP755548WBQ1
CSB-MP755548WBQ1
CSB-EP755548WBQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii tRNA modification GTPase MnmE (mnmE) CSB-YP755549PIJ
CSB-EP755549PIJ
CSB-BP755549PIJ
CSB-MP755549PIJ
CSB-EP755549PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Pyrrolidone-carboxylate peptidase 1 (pcp1) CSB-YP755550PIJ
CSB-EP755550PIJ
CSB-BP755550PIJ
CSB-MP755550PIJ
CSB-EP755550PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Aspartate--tRNA ligase (aspS), partial CSB-YP755551PIJ
CSB-EP755551PIJ
CSB-BP755551PIJ
CSB-MP755551PIJ
CSB-EP755551PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum 60 kDa chaperonin (groL), partial CSB-YP755552MWI
CSB-EP755552MWI
CSB-BP755552MWI
CSB-MP755552MWI
CSB-EP755552MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP755553GCI
CSB-EP755553GCI
CSB-BP755553GCI
CSB-MP755553GCI
CSB-EP755553GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Bifunctional protein GlmU (glmU) CSB-YP755554CKA
CSB-EP755554CKA
CSB-BP755554CKA
CSB-MP755554CKA
CSB-EP755554CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon (cheB2) CSB-YP755555VCQ
CSB-EP755555VCQ
CSB-BP755555VCQ
CSB-MP755555VCQ
CSB-EP755555VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Tryptophanase (tnaA) CSB-YP755556VCQ
CSB-EP755556VCQ
CSB-BP755556VCQ
CSB-MP755556VCQ
CSB-EP755556VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP755557VCQ
CSB-EP755557VCQ
CSB-BP755557VCQ
CSB-MP755557VCQ
CSB-EP755557VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus H (+)/Cl (-) exchange transporter ClcA (clcA), partial CSB-YP755558VCQ
CSB-EP755558VCQ
CSB-BP755558VCQ
CSB-MP755558VCQ
CSB-EP755558VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0271 protein VVA0778 (VVA0778) CSB-YP755559VCQ
CSB-EP755559VCQ
CSB-BP755559VCQ
CSB-MP755559VCQ
CSB-EP755559VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Glycine cleavage system H protein (gcvH) CSB-YP755560VCQ
CSB-EP755560VCQ
CSB-BP755560VCQ
CSB-MP755560VCQ
CSB-EP755560VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0267 protein VVA0554 (VVA0554) CSB-YP755561VCQ
CSB-EP755561VCQ
CSB-BP755561VCQ
CSB-MP755561VCQ
CSB-EP755561VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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