Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus subsp. pastoris 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP756183EYQ
CSB-EP756183EYQ
CSB-BP756183EYQ
CSB-MP756183EYQ
CSB-EP756183EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Phosphomethylpyrimidine synthase (thiC) CSB-YP756184EYQ
CSB-EP756184EYQ
CSB-BP756184EYQ
CSB-MP756184EYQ
CSB-EP756184EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 50S ribosomal protein L3 (rplC) CSB-YP756185EYQ
CSB-EP756185EYQ
CSB-BP756185EYQ
CSB-MP756185EYQ
CSB-EP756185EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 50S ribosomal protein L24 (rplX) CSB-YP756186EYQ
CSB-EP756186EYQ
CSB-BP756186EYQ
CSB-MP756186EYQ
CSB-EP756186EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Adenylate kinase (adk) CSB-YP756187EYQ
CSB-EP756187EYQ
CSB-BP756187EYQ
CSB-MP756187EYQ
CSB-EP756187EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Peptide chain release factor 1 (prfA) CSB-YP756188EYQ
CSB-EP756188EYQ
CSB-BP756188EYQ
CSB-MP756188EYQ
CSB-EP756188EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 30S ribosomal protein S10 (rpsJ) CSB-YP756189EYQ
CSB-EP756189EYQ
CSB-BP756189EYQ
CSB-MP756189EYQ
CSB-EP756189EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris DNA-directed RNA polymerase subunit gamma (rpoC1), partial CSB-YP756190EYQ
CSB-EP756190EYQ
CSB-BP756190EYQ
CSB-MP756190EYQ
CSB-EP756190EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris ATP synthase subunit b (atpF), partial CSB-YP756191EYQ1
CSB-EP756191EYQ1
CSB-BP756191EYQ1
CSB-MP756191EYQ1
CSB-EP756191EYQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP756192EYQ
CSB-EP756192EYQ
CSB-BP756192EYQ
CSB-MP756192EYQ
CSB-EP756192EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Probable cytosol aminopeptidase (pepA) CSB-YP756193EYQ
CSB-EP756193EYQ
CSB-BP756193EYQ
CSB-MP756193EYQ
CSB-EP756193EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Acetylornithine aminotransferase (argD) CSB-YP756194EYQ
CSB-EP756194EYQ
CSB-BP756194EYQ
CSB-MP756194EYQ
CSB-EP756194EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP756195EYQ
CSB-EP756195EYQ
CSB-BP756195EYQ
CSB-MP756195EYQ
CSB-EP756195EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Cysteine--tRNA ligase (cysS) CSB-YP756196EYQ
CSB-EP756196EYQ
CSB-BP756196EYQ
CSB-MP756196EYQ
CSB-EP756196EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Adaptive-response sensory-kinase sasA (sasA) CSB-YP756197EYQ
CSB-EP756197EYQ
CSB-BP756197EYQ
CSB-MP756197EYQ
CSB-EP756197EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Urease accessory protein UreG (ureG) CSB-YP756198EYQ
CSB-EP756198EYQ
CSB-BP756198EYQ
CSB-MP756198EYQ
CSB-EP756198EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Undecaprenyl-diphosphatase (uppP), partial CSB-YP756199EYQ
CSB-EP756199EYQ
CSB-BP756199EYQ
CSB-MP756199EYQ
CSB-EP756199EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Chaperone protein dnaK1 (dnaK1), partial CSB-YP756200EYQ
CSB-EP756200EYQ
CSB-BP756200EYQ
CSB-MP756200EYQ
CSB-EP756200EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase (acsF) CSB-YP756201EYQ
CSB-EP756201EYQ
CSB-BP756201EYQ
CSB-MP756201EYQ
CSB-EP756201EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Dihydroxy-acid dehydratase (ilvD), partial CSB-YP756202EYQ
CSB-EP756202EYQ
CSB-BP756202EYQ
CSB-MP756202EYQ
CSB-EP756202EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>