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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chromobacterium violaceum 50S ribosomal protein L36 (rpmJ) CSB-YP755822CKA
CSB-EP755822CKA
CSB-BP755822CKA
CSB-MP755822CKA
CSB-EP755822CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 8-amino-7-oxononanoate synthase (bioF) CSB-YP755823CKA
CSB-EP755823CKA
CSB-BP755823CKA
CSB-MP755823CKA
CSB-EP755823CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP755824CKA
CSB-EP755824CKA
CSB-BP755824CKA
CSB-MP755824CKA
CSB-EP755824CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Gamma-glutamyl phosphate reductase (proA) CSB-YP755825CKA
CSB-EP755825CKA
CSB-BP755825CKA
CSB-MP755825CKA
CSB-EP755825CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Methionyl-tRNA formyltransferase (fmt) CSB-YP755826CKA
CSB-EP755826CKA
CSB-BP755826CKA
CSB-MP755826CKA
CSB-EP755826CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Glutamate 5-kinase (proB) CSB-YP755827CKA
CSB-EP755827CKA
CSB-BP755827CKA
CSB-MP755827CKA
CSB-EP755827CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L4 (rplD) CSB-YP755828CKA
CSB-EP755828CKA
CSB-BP755828CKA
CSB-MP755828CKA
CSB-EP755828CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L16 (rplP) CSB-YP755829CKA
CSB-EP755829CKA
CSB-BP755829CKA
CSB-MP755829CKA
CSB-EP755829CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 50S ribosomal protein L6 (rplF) CSB-YP755830CKA
CSB-EP755830CKA
CSB-BP755830CKA
CSB-MP755830CKA
CSB-EP755830CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP755831CKA
CSB-EP755831CKA
CSB-BP755831CKA
CSB-MP755831CKA
CSB-EP755831CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Bis (5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) CSB-YP755832CKA
CSB-EP755832CKA
CSB-BP755832CKA
CSB-MP755832CKA
CSB-EP755832CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 60 kDa chaperonin 2 (groL2), partial CSB-YP755833CKA
CSB-EP755833CKA
CSB-BP755833CKA
CSB-MP755833CKA
CSB-EP755833CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Phosphoglucosamine mutase (glmM) CSB-YP755834CKA
CSB-EP755834CKA
CSB-BP755834CKA
CSB-MP755834CKA
CSB-EP755834CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP755835CKA
CSB-EP755835CKA
CSB-BP755835CKA
CSB-MP755835CKA
CSB-EP755835CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Phosphopentomutase (deoB) CSB-YP755836CKA
CSB-EP755836CKA
CSB-BP755836CKA
CSB-MP755836CKA
CSB-EP755836CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 5'-nucleotidase surE (surE) CSB-YP755837CKA
CSB-EP755837CKA
CSB-BP755837CKA
CSB-MP755837CKA
CSB-EP755837CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum 30S ribosomal protein S18 (rpsR) CSB-YP755838CKA
CSB-EP755838CKA
CSB-BP755838CKA
CSB-MP755838CKA
CSB-EP755838CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Lipoprotein signal peptidase (lspA), partial CSB-YP755839CKA1
CSB-EP755839CKA1
CSB-BP755839CKA1
CSB-MP755839CKA1
CSB-EP755839CKA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum GTPase Der (der) CSB-YP755840CKA
CSB-EP755840CKA
CSB-BP755840CKA
CSB-MP755840CKA
CSB-EP755840CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Flavohemoprotein (hmp) CSB-YP755841CKA
CSB-EP755841CKA
CSB-BP755841CKA
CSB-MP755841CKA
CSB-EP755841CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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