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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Anopheles gambiae 40S ribosomal protein S14a (RpS14a) CSB-YP755903BZL
CSB-EP755903BZL
CSB-BP755903BZL
CSB-MP755903BZL
CSB-EP755903BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae F-box/SPRY domain-containing protein 1 (Fsn) CSB-YP755904BZL
CSB-EP755904BZL
CSB-BP755904BZL
CSB-MP755904BZL
CSB-EP755904BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Protein mab-21-like (AGAP001033) CSB-YP755905BZL
CSB-EP755905BZL
CSB-BP755905BZL
CSB-MP755905BZL
CSB-EP755905BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Eukaryotic translation initiation factor 3 subunit G (eIF3-S4) CSB-YP755906BZL
CSB-EP755906BZL
CSB-BP755906BZL
CSB-MP755906BZL
CSB-EP755906BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calcium-dependent protein kinase 3 (CPK3), partial CSB-YP755907PMT
CSB-EP755907PMT
CSB-BP755907PMT
CSB-MP755907PMT
CSB-EP755907PMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium yoelii yoelii Translation factor GUF1 homolog, mitochondrial (PY03426), partial CSB-YP755908PMT
CSB-EP755908PMT
CSB-BP755908PMT
CSB-MP755908PMT
CSB-EP755908PMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium yoelii yoelii 40S ribosomal protein S3a (PY02291) CSB-YP755909PMT
CSB-EP755909PMT
CSB-BP755909PMT
CSB-MP755909PMT
CSB-EP755909PMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ornithine aminotransferase (OAT) CSB-YP755910PMT
CSB-EP755910PMT
CSB-BP755910PMT
CSB-MP755910PMT
CSB-EP755910PMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Class A basic helix-loop-helix protein 15 (BHLHA15) CSB-YP755911HU
CSB-EP755911HU
CSB-BP755911HU
CSB-MP755911HU
CSB-EP755911HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein SSX7 (SSX7) CSB-YP755912HU
CSB-EP755912HU
CSB-BP755912HU
CSB-MP755912HU
CSB-EP755912HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Achaete-scute homolog 5 (ASCL5) CSB-YP755913HU
CSB-EP755913HU
CSB-BP755913HU
CSB-MP755913HU
CSB-EP755913HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Monocarboxylate transporter 14 (SLC16A14), partial CSB-YP755914HU
CSB-EP755914HU
CSB-BP755914HU
CSB-MP755914HU
CSB-EP755914HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative ubiquitin carboxyl-terminal hydrolase 17-like protein 1 (USP17L1P) CSB-YP755915HU
CSB-EP755915HU
CSB-BP755915HU
CSB-MP755915HU
CSB-EP755915HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Orotate phosphoribosyltransferase (ura-5) CSB-YP755916NHA
CSB-EP755916NHA
CSB-BP755916NHA
CSB-MP755916NHA
CSB-EP755916NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa 5-aminolevulinate synthase, mitochondrial (hem-1), partial CSB-YP755917NHA
CSB-EP755917NHA
CSB-BP755917NHA
CSB-MP755917NHA
CSB-EP755917NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Iron-sulfur clusters transporter atm-1, mitochondrial (atm-1), partial CSB-YP755918NHA1
CSB-EP755918NHA1
CSB-BP755918NHA1
CSB-MP755918NHA1
CSB-EP755918NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mediator of RNA polymerase II transcription subunit 10 (nut-2) CSB-YP755919NHA
CSB-EP755919NHA
CSB-BP755919NHA
CSB-MP755919NHA
CSB-EP755919NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa COP9 signalosome complex subunit 5 (rri-1) CSB-YP755920NHA
CSB-EP755920NHA
CSB-BP755920NHA
CSB-MP755920NHA
CSB-EP755920NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Polyadenylation factor subunit 2 (pfs-2), partial CSB-YP755921NHA
CSB-EP755921NHA
CSB-BP755921NHA
CSB-MP755921NHA
CSB-EP755921NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa SWR1-complex protein 5 (swc-5) CSB-YP755922NHA
CSB-EP755922NHA
CSB-BP755922NHA
CSB-MP755922NHA
CSB-EP755922NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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