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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian Transcription elongation factor GreA (greA) CSB-YP757025BAAE
CSB-EP757025BAAE
CSB-BP757025BAAE
CSB-MP757025BAAE
CSB-EP757025BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Shikimate dehydrogenase (aroE) CSB-YP757026BAAE
CSB-EP757026BAAE
CSB-BP757026BAAE
CSB-MP757026BAAE
CSB-EP757026BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Heat-inducible transcription repressor HrcA (hrcA) CSB-YP757027BAAE
CSB-EP757027BAAE
CSB-BP757027BAAE
CSB-MP757027BAAE
CSB-EP757027BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian DNA repair protein recO (recO) CSB-YP757028BAAE
CSB-EP757028BAAE
CSB-BP757028BAAE
CSB-MP757028BAAE
CSB-EP757028BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase 2 (murG2) CSB-YP757029BAAE
CSB-EP757029BAAE
CSB-BP757029BAAE
CSB-MP757029BAAE
CSB-EP757029BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Elongation factor P (efp) CSB-YP757030BAAE
CSB-EP757030BAAE
CSB-BP757030BAAE
CSB-MP757030BAAE
CSB-EP757030BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Acetylglutamate kinase (argB) CSB-YP757031BAAE
CSB-EP757031BAAE
CSB-BP757031BAAE
CSB-MP757031BAAE
CSB-EP757031BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Segregation and condensation protein A (scpA) CSB-YP757032BAAE
CSB-EP757032BAAE
CSB-BP757032BAAE
CSB-MP757032BAAE
CSB-EP757032BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP757033BAAE
CSB-EP757033BAAE
CSB-BP757033BAAE
CSB-MP757033BAAE
CSB-EP757033BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP757034BAAE
CSB-EP757034BAAE
CSB-BP757034BAAE
CSB-MP757034BAAE
CSB-EP757034BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP757035BAAE
CSB-EP757035BAAE
CSB-BP757035BAAE
CSB-MP757035BAAE
CSB-EP757035BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP757036BAAE
CSB-EP757036BAAE
CSB-BP757036BAAE
CSB-MP757036BAAE
CSB-EP757036BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S16 (rpsP) CSB-YP757037BAAE
CSB-EP757037BAAE
CSB-BP757037BAAE
CSB-MP757037BAAE
CSB-EP757037BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Elongation factor Ts (tsf) CSB-YP757038BAAE
CSB-EP757038BAAE
CSB-BP757038BAAE
CSB-MP757038BAAE
CSB-EP757038BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 1-deoxy-D-xylulose 5-phosphate reductoisomerase 2 (dxr2) CSB-YP757039BAAE
CSB-EP757039BAAE
CSB-BP757039BAAE
CSB-MP757039BAAE
CSB-EP757039BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Putative competence-damage inducible protein (cinA) CSB-YP757040BAAE
CSB-EP757040BAAE
CSB-BP757040BAAE
CSB-MP757040BAAE
CSB-EP757040BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian tRNA dimethylallyltransferase (miaA) CSB-YP757041BAAE
CSB-EP757041BAAE
CSB-BP757041BAAE
CSB-MP757041BAAE
CSB-EP757041BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Glycerol-3-phosphate acyltransferase 2 (plsY2), partial CSB-YP757042BAAE1
CSB-EP757042BAAE1
CSB-BP757042BAAE1
CSB-MP757042BAAE1
CSB-EP757042BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0316 protein BT9727_3154 (BT9727_3154), partial CSB-YP757043BAAE1
CSB-EP757043BAAE1
CSB-BP757043BAAE1
CSB-MP757043BAAE1
CSB-EP757043BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0178 protein BT9727_2823 (BT9727_2823) CSB-YP757044BAAE
CSB-EP757044BAAE
CSB-BP757044BAAE
CSB-MP757044BAAE
CSB-EP757044BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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