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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella putrefaciens Phosphoserine aminotransferase (serC) CSB-YP018838STQ
CSB-EP018838STQ
CSB-BP018838STQ
CSB-MP018838STQ
CSB-EP018838STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Glycine cleavage system H protein (gcvH) CSB-YP009335STQ
CSB-EP009335STQ
CSB-BP009335STQ
CSB-MP009335STQ
CSB-EP009335STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Methylated-DNA--protein-cysteine methyltransferase (ogt) CSB-YP013788MNM
CSB-EP013788MNM
CSB-BP013788MNM
CSB-MP013788MNM
CSB-EP013788MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallosphaera sedula Protein pelota homolog (pelA) CSB-YP017778MNM
CSB-EP017778MNM
CSB-BP017778MNM
CSB-MP017778MNM
CSB-EP017778MNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Translation initiation factor IF-2 (infB), partial CSB-YP015164BPF
CSB-EP015164BPF
CSB-BP015164BPF
CSB-MP015164BPF
CSB-EP015164BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Malate dehydrogenase (mdh) CSB-YP013623BPF
CSB-EP013623BPF
CSB-BP013623BPF
CSB-MP013623BPF
CSB-EP013623BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium sp. Thymidylate kinase (tmk) CSB-YP007226BPF
CSB-EP007226BPF
CSB-BP007226BPF
CSB-MP007226BPF
CSB-EP007226BPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Mannose-6-phosphate isomerase (MPI) CSB-YP014754EQV
CSB-EP014754EQV
CSB-BP014754EQV
CSB-MP014754EQV
CSB-EP014754EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Sodium channel subunit beta-1 (SCN1B), partial CSB-YP020835EQV1
CSB-EP020835EQV1
CSB-BP020835EQV1
CSB-MP020835EQV1
CSB-EP020835EQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Eukaryotic initiation factor 4A-I (EIF4A1) CSB-YP007550EQV
CSB-EP007550EQV
CSB-BP007550EQV
CSB-MP007550EQV
CSB-EP007550EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Glypican-3 (GPC3), partial CSB-YP009705EQV
CSB-EP009705EQV
CSB-BP009705EQV
CSB-EP009705EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orientia tsutsugamushi ATP synthase subunit beta (atpD) CSB-YP002350ODJ
CSB-EP002350ODJ
CSB-BP002350ODJ
CSB-MP002350ODJ
CSB-EP002350ODJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. michiganensis Serine hydroxymethyltransferase (glyA) CSB-YP021274CWT
CSB-EP021274CWT
CSB-BP021274CWT
CSB-MP021274CWT
CSB-EP021274CWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Ribonuclease 3 (rnc) CSB-YP019809PZM
CSB-EP019809PZM
CSB-BP019809PZM
CSB-MP019809PZM
CSB-EP019809PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Thymidylate kinase (tmk) CSB-YP007226PZM
CSB-EP007226PZM
CSB-BP007226PZM
CSB-MP007226PZM
CSB-EP007226PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina Cysteine desulfurase (iscS) CSB-YP015769PZM
CSB-EP015769PZM
CSB-BP015769PZM
CSB-MP015769PZM
CSB-EP015769PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas mendocina ATP synthase subunit beta (atpD) CSB-YP002350PZM
CSB-EP002350PZM
CSB-BP002350PZM
CSB-MP002350PZM
CSB-EP002350PZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens Oligoribonuclease (orn) CSB-YP019586STQ
CSB-EP019586STQ
CSB-BP019586STQ
CSB-MP019586STQ
CSB-EP019586STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens L-threonine 3-dehydrogenase (tdh) CSB-YP023350STQ
CSB-EP023350STQ
CSB-BP023350STQ
CSB-MP023350STQ
CSB-EP023350STQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella putrefaciens ATP synthase subunit a (atpB), partial CSB-YP015070STQ1
CSB-EP015070STQ1
CSB-BP015070STQ1
CSB-MP015070STQ1
CSB-EP015070STQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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