Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Photobacterium profundum Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP760934PIG
CSB-EP760934PIG
CSB-BP760934PIG
CSB-MP760934PIG
CSB-EP760934PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP760935PIG
CSB-EP760935PIG
CSB-BP760935PIG
CSB-MP760935PIG
CSB-EP760935PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Protein RecA (recA) CSB-YP760936PIG
CSB-EP760936PIG
CSB-BP760936PIG
CSB-MP760936PIG
CSB-EP760936PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP760937PIG
CSB-EP760937PIG
CSB-BP760937PIG
CSB-MP760937PIG
CSB-EP760937PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP760938PIG
CSB-EP760938PIG
CSB-BP760938PIG
CSB-MP760938PIG
CSB-EP760938PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Elongation factor Ts (tsf) CSB-YP760939PIG
CSB-EP760939PIG
CSB-BP760939PIG
CSB-MP760939PIG
CSB-EP760939PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Lipid-A-disaccharide synthase (lpxB) CSB-YP760940PIG
CSB-EP760940PIG
CSB-BP760940PIG
CSB-MP760940PIG
CSB-EP760940PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UPF0253 protein PBPRA2947 (PBPRA2947) CSB-YP760941PIG
CSB-EP760941PIG
CSB-BP760941PIG
CSB-MP760941PIG
CSB-EP760941PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Uracil phosphoribosyltransferase (upp) CSB-YP760942PIG
CSB-EP760942PIG
CSB-BP760942PIG
CSB-MP760942PIG
CSB-EP760942PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Probable rRNA maturation factor (PBPRA2880) CSB-YP760943PIG
CSB-EP760943PIG
CSB-BP760943PIG
CSB-MP760943PIG
CSB-EP760943PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Peptide chain release factor 1 (prfA) CSB-YP760944PIG
CSB-EP760944PIG
CSB-BP760944PIG
CSB-MP760944PIG
CSB-EP760944PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Pyridoxamine kinase (pdxY) CSB-YP760945PIG
CSB-EP760945PIG
CSB-BP760945PIG
CSB-MP760945PIG
CSB-EP760945PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Tryptophan synthase beta chain (trpB) CSB-YP760946PIG
CSB-EP760946PIG
CSB-BP760946PIG
CSB-MP760946PIG
CSB-EP760946PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UPF0283 membrane protein PBPRA2435 (PBPRA2435), partial CSB-YP760947PIG1
CSB-EP760947PIG1
CSB-BP760947PIG1
CSB-MP760947PIG1
CSB-EP760947PIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP760948PIG
CSB-EP760948PIG
CSB-BP760948PIG
CSB-MP760948PIG
CSB-EP760948PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP760949PIG
CSB-EP760949PIG
CSB-BP760949PIG
CSB-MP760949PIG
CSB-EP760949PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Multidrug resistance protein norM (norM), partial CSB-YP760950PIG
CSB-EP760950PIG
CSB-BP760950PIG
CSB-MP760950PIG
CSB-EP760950PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP760951PIG
CSB-EP760951PIG
CSB-BP760951PIG
CSB-MP760951PIG
CSB-EP760951PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Periplasmic nitrate reductase 2 (napA2), partial CSB-YP760952PIG
CSB-EP760952PIG
CSB-BP760952PIG
CSB-MP760952PIG
CSB-EP760952PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum NAD-dependent malic enzyme 1 (maeA1), partial CSB-YP760953PIG
CSB-EP760953PIG
CSB-BP760953PIG
CSB-MP760953PIG
CSB-EP760953PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>