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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nanoarchaeum equitans Elongation factor 1-alpha (tuf) CSB-YP762156NAQ
CSB-EP762156NAQ
CSB-BP762156NAQ
CSB-MP762156NAQ
CSB-EP762156NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans tRNA-splicing endonuclease (endA) CSB-YP762157NAQ
CSB-EP762157NAQ
CSB-BP762157NAQ
CSB-MP762157NAQ
CSB-EP762157NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans 50S ribosomal protein L32e (rpl32e) CSB-YP762158NAQ
CSB-EP762158NAQ
CSB-BP762158NAQ
CSB-MP762158NAQ
CSB-EP762158NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans 30S ribosomal protein S19P (rps19p) CSB-YP762159NAQ
CSB-EP762159NAQ
CSB-BP762159NAQ
CSB-MP762159NAQ
CSB-EP762159NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans 30S ribosomal protein S11P (rps11p) CSB-YP762160NAQ
CSB-EP762160NAQ
CSB-BP762160NAQ
CSB-MP762160NAQ
CSB-EP762160NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans Probable peroxiredoxin (NEQ191) CSB-YP762161NAQ
CSB-EP762161NAQ
CSB-BP762161NAQ
CSB-MP762161NAQ
CSB-EP762161NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nanoarchaeum equitans 30S ribosomal protein S17P (rps17p) CSB-YP762162NAQ
CSB-EP762162NAQ
CSB-BP762162NAQ
CSB-MP762162NAQ
CSB-EP762162NAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autoinducer 2-binding protein lsrB (lsrB) CSB-YP762163YAS
CSB-EP762163YAS
CSB-BP762163YAS
CSB-MP762163YAS
CSB-EP762163YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maltose transport system permease protein malF (malF), partial CSB-YP762164YAS
CSB-EP762164YAS
CSB-BP762164YAS
CSB-MP762164YAS
CSB-EP762164YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP762165YAS1
CSB-EP762165YAS1
CSB-BP762165YAS1
CSB-MP762165YAS1
CSB-EP762165YAS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP762166YAS
CSB-EP762166YAS
CSB-BP762166YAS
CSB-MP762166YAS
CSB-EP762166YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Putative DNA helicase INO80 (INO80), partial CSB-YP762167DOT
CSB-EP762167DOT
CSB-BP762167DOT
CSB-MP762167DOT
CSB-EP762167DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Serine/threonine-protein kinase BUD32 (BUD32) CSB-YP762168DOT
CSB-EP762168DOT
CSB-BP762168DOT
CSB-MP762168DOT
CSB-EP762168DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent RNA helicase DBP9 (DBP9), partial CSB-YP762169DOT
CSB-EP762169DOT
CSB-BP762169DOT
CSB-MP762169DOT
CSB-EP762169DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Translation factor GUF1, mitochondrial (GUF1), partial CSB-YP762170DOT
CSB-EP762170DOT
CSB-BP762170DOT
CSB-MP762170DOT
CSB-EP762170DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Vacuolar protein-sorting protein BRO1 (BRO1), partial CSB-YP762171DOT
CSB-EP762171DOT
CSB-BP762171DOT
CSB-MP762171DOT
CSB-EP762171DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Histone H2A.1 (HTA1) CSB-YP762172DOT
CSB-EP762172DOT
CSB-BP762172DOT
CSB-MP762172DOT
CSB-EP762172DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii RNA polymerase II transcription factor B subunit 5 (TFB5) CSB-YP762173DOT
CSB-EP762173DOT
CSB-BP762173DOT
CSB-MP762173DOT
CSB-EP762173DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Arginase (CAR1) CSB-YP762174DOT
CSB-EP762174DOT
CSB-BP762174DOT
CSB-MP762174DOT
CSB-EP762174DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype C Gag-Pol polyprotein (gag-pol), partial CSB-YP762175HDAH
CSB-EP762175HDAH
CSB-BP762175HDAH
CSB-MP762175HDAH
CSB-EP762175HDAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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