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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Glutamate 5-kinase (proB) CSB-YP761956BQN
CSB-EP761956BQN
CSB-BP761956BQN
CSB-MP761956BQN
CSB-EP761956BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Type II pantothenate kinase (coaW) CSB-YP761957BQN
CSB-EP761957BQN
CSB-BP761957BQN
CSB-MP761957BQN
CSB-EP761957BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribose-5-phosphate isomerase A (rpiA) CSB-YP761958BQN
CSB-EP761958BQN
CSB-BP761958BQN
CSB-MP761958BQN
CSB-EP761958BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative metal-dependent hydrolase BCE_2729 (BCE_2729) CSB-YP761959BQN
CSB-EP761959BQN
CSB-BP761959BQN
CSB-MP761959BQN
CSB-EP761959BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Thymidylate synthase (thyA) CSB-YP761960BQN
CSB-EP761960BQN
CSB-BP761960BQN
CSB-MP761960BQN
CSB-EP761960BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Acetaldehyde dehydrogenase (BCE_2156) CSB-YP761961BQN
CSB-EP761961BQN
CSB-BP761961BQN
CSB-MP761961BQN
CSB-EP761961BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein H 2 (sspH2) CSB-YP761962BQN
CSB-EP761962BQN
CSB-BP761962BQN
CSB-MP761962BQN
CSB-EP761962BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP761963BQN
CSB-EP761963BQN
CSB-BP761963BQN
CSB-MP761963BQN
CSB-EP761963BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-dehydroquinate synthase (aroB) CSB-YP761964BQN
CSB-EP761964BQN
CSB-BP761964BQN
CSB-MP761964BQN
CSB-EP761964BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Sulfate adenylyltransferase (sat) CSB-YP761965BQN
CSB-EP761965BQN
CSB-BP761965BQN
CSB-MP761965BQN
CSB-EP761965BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP761966BQN
CSB-EP761966BQN
CSB-BP761966BQN
CSB-MP761966BQN
CSB-EP761966BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable inorganic polyphosphate/ATP-NAD kinase 1 (ppnK1) CSB-YP761967BQN
CSB-EP761967BQN
CSB-BP761967BQN
CSB-MP761967BQN
CSB-EP761967BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ferrochelatase 1 (hemH1) CSB-YP761968BQN
CSB-EP761968BQN
CSB-BP761968BQN
CSB-MP761968BQN
CSB-EP761968BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP761969BQN
CSB-EP761969BQN
CSB-BP761969BQN
CSB-MP761969BQN
CSB-EP761969BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Thiazole synthase (thiG) CSB-YP761970BQN
CSB-EP761970BQN
CSB-BP761970BQN
CSB-MP761970BQN
CSB-EP761970BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribose import ATP-binding protein RbsA (rbsA) CSB-YP761971BQN
CSB-EP761971BQN
CSB-BP761971BQN
CSB-MP761971BQN
CSB-EP761971BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Regulatory protein recX (recX) CSB-YP761972BQN
CSB-EP761972BQN
CSB-BP761972BQN
CSB-MP761972BQN
CSB-EP761972BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Arginine regulator (argR1) CSB-YP761973BQN
CSB-EP761973BQN
CSB-BP761973BQN
CSB-MP761973BQN
CSB-EP761973BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP761974BQN
CSB-EP761974BQN
CSB-BP761974BQN
CSB-MP761974BQN
CSB-EP761974BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP761975BQN
CSB-EP761975BQN
CSB-BP761975BQN
CSB-MP761975BQN
CSB-EP761975BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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