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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pan troglodytes Sperm protein associated with the nucleus on the X chromosome N1 (SPANXN1) CSB-YP761435EQV
CSB-EP761435EQV
CSB-BP761435EQV
CSB-MP761435EQV
CSB-EP761435EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse TATA box-binding protein-like protein 2 (Tbpl2) CSB-YP761436MO
CSB-EP761436MO
CSB-BP761436MO
CSB-MP761436MO
CSB-EP761436MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Acyl-coenzyme A synthetase ACSM3, mitochondrial (Acsm3) CSB-YP761437RA
CSB-EP761437RA
CSB-BP761437RA
CSB-MP761437RA
CSB-EP761437RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Atherin (SAMD1) CSB-YP761438HU
CSB-EP761438HU
CSB-BP761438HU
CSB-MP761438HU
CSB-EP761438HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus G-protein coupled receptor homolog UL33 (UL33), partial CSB-YP761439HWW1
CSB-EP761439HWW1
CSB-BP761439HWW1
CSB-MP761439HWW1
CSB-EP761439HWW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum S-adenosylmethionine synthase 2 (SAMS2) CSB-YP761440NHE
CSB-EP761440NHE
CSB-BP761440NHE
CSB-MP761440NHE
CSB-EP761440NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Mastermind-like protein 1 (Maml1), partial CSB-YP761441MO
CSB-EP761441MO
CSB-BP761441MO
CSB-MP761441MO
CSB-EP761441MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Cu-Zn] (SOD1) CSB-YP761442PHAZ
CSB-EP761442PHAZ
CSB-BP761442PHAZ
CSB-MP761442PHAZ
CSB-EP761442PHAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hippocampus comes Cellular retinoic acid-binding protein 1 (crabp1) CSB-YP761443HHF
CSB-EP761443HHF
CSB-BP761443HHF
CSB-MP761443HHF
CSB-EP761443HHF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bitis gabonica L-amino-acid oxidase CSB-YP761444BGW
CSB-EP761444BGW
CSB-BP761444BGW
CSB-MP761444BGW
CSB-EP761444BGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bitis gabonica Protease inhibitor bitisilin-2 CSB-YP761445BGW
CSB-EP761445BGW
CSB-BP761445BGW
CSB-MP761445BGW
CSB-EP761445BGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bitis gabonica Protease inhibitor bitisilin-1 CSB-YP761446BGW
CSB-EP761446BGW
CSB-BP761446BGW
CSB-MP761446BGW
CSB-EP761446BGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Telethonin (TCAP) CSB-YP761447BO
CSB-EP761447BO
CSB-BP761447BO
CSB-MP761447BO
CSB-EP761447BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Vomeronasal type-2 receptor 26 (Vmn2r26), partial CSB-YP761448MO
CSB-EP761448MO
CSB-BP761448MO
CSB-MP761448MO
CSB-EP761448MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erignathus barbatus Sex-determining region Y protein (SRY) CSB-YP761449EBAN
CSB-EP761449EBAN
CSB-BP761449EBAN
CSB-MP761449EBAN
CSB-EP761449EBAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mirounga leonina Sex-determining region Y protein (SRY) CSB-YP761450MHA
CSB-EP761450MHA
CSB-BP761450MHA
CSB-MP761450MHA
CSB-EP761450MHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callorhinus ursinus Sex-determining region Y protein (SRY) CSB-YP761451CBE
CSB-EP761451CBE
CSB-BP761451CBE
CSB-MP761451CBE
CSB-EP761451CBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actin (ACT) CSB-YP761452GCAG
CSB-EP761452GCAG
CSB-BP761452GCAG
CSB-MP761452GCAG
CSB-EP761452GCAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Progestin and adipoQ receptor family member 3 (Paqr3), partial CSB-YP761453MO1
CSB-EP761453MO1
CSB-BP761453MO1
CSB-MP761453MO1
CSB-EP761453MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio E3 ubiquitin-protein ligase MYLIP-A (mylipa) CSB-YP761454DIL
CSB-EP761454DIL
CSB-BP761454DIL
CSB-MP761454DIL
CSB-EP761454DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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