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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio vulnificus tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP762658VCQ
CSB-EP762658VCQ
CSB-BP762658VCQ
CSB-MP762658VCQ
CSB-EP762658VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD), partial CSB-YP762659VCQ
CSB-EP762659VCQ
CSB-BP762659VCQ
CSB-MP762659VCQ
CSB-EP762659VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 50S ribosomal protein L7/L12 (rplL) CSB-YP762660VCQ
CSB-EP762660VCQ
CSB-BP762660VCQ
CSB-MP762660VCQ
CSB-EP762660VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Acetyl-coenzyme A synthetase (acsA), partial CSB-YP762661VCQ
CSB-EP762661VCQ
CSB-BP762661VCQ
CSB-MP762661VCQ
CSB-EP762661VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Uncharacterized protein YjeA (yjeA) CSB-YP762662VCQ
CSB-EP762662VCQ
CSB-BP762662VCQ
CSB-MP762662VCQ
CSB-EP762662VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein hfq (hfq) CSB-YP762663VCQ
CSB-EP762663VCQ
CSB-BP762663VCQ
CSB-MP762663VCQ
CSB-EP762663VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Acetylornithine aminotransferase (argD) CSB-YP762664VCQ
CSB-EP762664VCQ
CSB-BP762664VCQ
CSB-MP762664VCQ
CSB-EP762664VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Elongation factor Tu 1 (tuf1) CSB-YP762665VCQ
CSB-EP762665VCQ
CSB-BP762665VCQ
CSB-MP762665VCQ
CSB-EP762665VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP762666VCQ
CSB-EP762666VCQ
CSB-BP762666VCQ
CSB-MP762666VCQ
CSB-EP762666VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Primosomal replication protein n (priB) CSB-YP762667VCQ
CSB-EP762667VCQ
CSB-BP762667VCQ
CSB-MP762667VCQ
CSB-EP762667VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UvrABC system protein A (uvrA), partial CSB-YP762668VCQ
CSB-EP762668VCQ
CSB-BP762668VCQ
CSB-MP762668VCQ
CSB-EP762668VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Valine--tRNA ligase (valS), partial CSB-YP762669VCQ
CSB-EP762669VCQ
CSB-BP762669VCQ
CSB-MP762669VCQ
CSB-EP762669VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0149 protein VV2847 (VV2847) CSB-YP762670VCQ
CSB-EP762670VCQ
CSB-BP762670VCQ
CSB-MP762670VCQ
CSB-EP762670VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD (rlmD) CSB-YP762671VCQ
CSB-EP762671VCQ
CSB-BP762671VCQ
CSB-MP762671VCQ
CSB-EP762671VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP762672VCQ
CSB-EP762672VCQ
CSB-BP762672VCQ
CSB-MP762672VCQ
CSB-EP762672VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus S-ribosylhomocysteine lyase (luxS) CSB-YP762673VCQ
CSB-EP762673VCQ
CSB-BP762673VCQ
CSB-MP762673VCQ
CSB-EP762673VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP762674VCQ
CSB-EP762674VCQ
CSB-BP762674VCQ
CSB-MP762674VCQ
CSB-EP762674VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP762675VCQ
CSB-EP762675VCQ
CSB-BP762675VCQ
CSB-MP762675VCQ
CSB-EP762675VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Peptide chain release factor 3 (prfC), partial CSB-YP762676VCQ
CSB-EP762676VCQ
CSB-BP762676VCQ
CSB-MP762676VCQ
CSB-EP762676VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Amino-acid acetyltransferase (argA) CSB-YP762677VCQ
CSB-EP762677VCQ
CSB-BP762677VCQ
CSB-MP762677VCQ
CSB-EP762677VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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