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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Takifugu rubripes Muscleblind-like protein 2a (mbnl2a) CSB-YP761375TKY
CSB-EP761375TKY
CSB-BP761375TKY
CSB-MP761375TKY
CSB-EP761375TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio 60S ribosomal protein L36 (rpl36) CSB-YP761376DIL
CSB-EP761376DIL
CSB-BP761376DIL
CSB-MP761376DIL
CSB-EP761376DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydra vulgaris Bifunctional arginine demethylase and lysyl-hydroxylase PSR (PSR) CSB-YP761377HXN
CSB-EP761377HXN
CSB-BP761377HXN
CSB-MP761377HXN
CSB-EP761377HXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YMR031W-A (YMR031W-A) CSB-YP761378SVG
CSB-EP761378SVG
CSB-BP761378SVG
CSB-MP761378SVG
CSB-EP761378SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae MEC1-mediated checkpoint protein HUG1 (HUG1) CSB-YP761379SVG
CSB-EP761379SVG
CSB-BP761379SVG
CSB-MP761379SVG
CSB-EP761379SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative viral protein-binding protein C1 CSB-YP761380HU
CSB-EP761380HU
CSB-BP761380HU
CSB-MP761380HU
CSB-EP761380HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica MADS-box transcription factor 15 (MADS15) CSB-YP761381OFG
CSB-EP761381OFG
CSB-BP761381OFG
CSB-MP761381OFG
CSB-EP761381OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig 40S ribosomal protein S28 (RPS28) CSB-YP761382PI
CSB-EP761382PI
CSB-BP761382PI
CSB-MP761382PI
CSB-EP761382PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Castanea sativa Photosystem I reaction center subunit VIII (psaI), partial CSB-YP761383CEL
CSB-EP761383CEL
CSB-BP761383CEL
CSB-MP761383CEL
CSB-EP761383CEL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sphingolipid delta (4)-desaturase/C4-hydroxylase DES2 (DEGS2), partial CSB-YP761384HU1
CSB-EP761384HU1
CSB-BP761384HU1
CSB-MP761384HU1
CSB-EP761384HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat N-alpha-acetyltransferase 25, NatB auxiliary subunit (Naa25), partial CSB-YP761385RA
CSB-EP761385RA
CSB-BP761385RA
CSB-MP761385RA
CSB-EP761385RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endo-1,4-beta-xylanase 6 (XYN6) CSB-YP761386DOZ
CSB-EP761386DOZ
CSB-BP761386DOZ
CSB-MP761386DOZ
CSB-EP761386DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Cathelicidin-1 (CATHL1) CSB-YP761387CH
CSB-EP761387CH
CSB-BP761387CH
CSB-MP761387CH
CSB-EP761387CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chinchilla lanigera DNA-directed RNA polymerases I, II, and III subunit RPABC5 (POLR2L) CSB-YP761388CIL
CSB-EP761388CIL
CSB-BP761388CIL
CSB-MP761388CIL
CSB-EP761388CIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Type III iodothyronine deiodinase (DIO3), partial CSB-YP761389PI
CSB-EP761389PI
CSB-BP761389PI
CSB-MP761389PI
CSB-EP761389PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eimeria tenella Casein kinase I CSB-YP761390EJU
CSB-EP761390EJU
CSB-BP761390EJU
CSB-MP761390EJU
CSB-EP761390EJU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GATA transcription factor 19 (GATA19) CSB-YP761391DOA
CSB-EP761391DOA
CSB-BP761391DOA
CSB-MP761391DOA
CSB-EP761391DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calycanthus floridus Maturase K (matK), partial CSB-YP761392CBL
CSB-EP761392CBL
CSB-BP761392CBL
CSB-MP761392CBL
CSB-EP761392CBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Telomere repeat-binding protein 5 (TRP5), partial CSB-YP761393DOA
CSB-EP761393DOA
CSB-BP761393DOA
CSB-MP761393DOA
CSB-EP761393DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Guanine nucleotide-binding protein G (s) subunit alpha isoforms XLas (Gnas), partial CSB-YP761394MO
CSB-EP761394MO
CSB-BP761394MO
CSB-MP761394MO
CSB-EP761394MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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