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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP763961SKW
CSB-EP763961SKW
CSB-BP763961SKW
CSB-MP763961SKW
CSB-EP763961SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0133 protein SAS0436 (SAS0436) CSB-YP763962SKW
CSB-EP763962SKW
CSB-BP763962SKW
CSB-MP763962SKW
CSB-EP763962SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized lipoprotein SAS0403 (SAS0403) CSB-YP763963SKW
CSB-EP763963SKW
CSB-BP763963SKW
CSB-MP763963SKW
CSB-EP763963SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Xanthine phosphoribosyltransferase (xpt) CSB-YP763964SKW
CSB-EP763964SKW
CSB-BP763964SKW
CSB-MP763964SKW
CSB-EP763964SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Single-stranded DNA-binding protein 2 (ssb2) CSB-YP763965SKW
CSB-EP763965SKW
CSB-BP763965SKW
CSB-MP763965SKW
CSB-EP763965SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP763966SKW
CSB-EP763966SKW
CSB-BP763966SKW
CSB-MP763966SKW
CSB-EP763966SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein esaC (esaC) CSB-YP763967SKW
CSB-EP763967SKW
CSB-BP763967SKW
CSB-MP763967SKW
CSB-EP763967SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ESAT-6 secretion system extracellular protein A (esxA) CSB-YP763968SKW
CSB-BP763968SKW
CSB-MP763968SKW
CSB-EP763968SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus D-ribose pyranase (rbsD) CSB-YP763969SKW
CSB-EP763969SKW
CSB-BP763969SKW
CSB-MP763969SKW
CSB-EP763969SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Iron-sulfur cluster repair protein ScdA (scdA) CSB-YP763970SKW
CSB-EP763970SKW
CSB-BP763970SKW
CSB-MP763970SKW
CSB-EP763970SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Formate acetyltransferase (pflB), partial CSB-YP763971SKW
CSB-EP763971SKW
CSB-BP763971SKW
CSB-MP763971SKW
CSB-EP763971SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP763972SKW
CSB-EP763972SKW
CSB-BP763972SKW
CSB-MP763972SKW
CSB-EP763972SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative aldehyde dehydrogenase AldA (aldA) CSB-YP763973SKW
CSB-EP763973SKW
CSB-BP763973SKW
CSB-MP763973SKW
CSB-EP763973SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized lipoprotein SAS0074 (SAS0074) CSB-YP763974SKW
CSB-EP763974SKW
CSB-BP763974SKW
CSB-MP763974SKW
CSB-EP763974SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chromosomal replication initiator protein DnaA (dnaA) CSB-YP763975SKW
CSB-EP763975SKW
CSB-BP763975SKW
CSB-MP763975SKW
CSB-EP763975SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA modification GTPase MnmE (mnmE) CSB-YP763976SKV
CSB-EP763976SKV
CSB-BP763976SKV
CSB-MP763976SKV
CSB-EP763976SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0312 protein SAR2769 (SAR2769) CSB-YP763977SKV
CSB-EP763977SKV
CSB-BP763977SKV
CSB-MP763977SKV
CSB-EP763977SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP763978SKV
CSB-EP763978SKV
CSB-BP763978SKV
CSB-MP763978SKV
CSB-EP763978SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein translocase subunit SecA 2 (secA2), partial CSB-YP763979SKV
CSB-EP763979SKV
CSB-BP763979SKV
CSB-MP763979SKV
CSB-EP763979SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator ArcR (arcR) CSB-YP763980SKV
CSB-EP763980SKV
CSB-BP763980SKV
CSB-MP763980SKV
CSB-EP763980SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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