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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photobacterium profundum ATP synthase subunit delta 2 (atpH2) CSB-YP764361PIG
CSB-EP764361PIG
CSB-BP764361PIG
CSB-MP764361PIG
CSB-EP764361PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Acetate kinase 2 (ackA2) CSB-YP764362PIG
CSB-EP764362PIG
CSB-BP764362PIG
CSB-MP764362PIG
CSB-EP764362PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum ATP synthase subunit a 1 (atpB1), partial CSB-YP764363PIG1
CSB-EP764363PIG1
CSB-BP764363PIG1
CSB-MP764363PIG1
CSB-EP764363PIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum ATP synthase subunit alpha 1 (atpA1), partial CSB-YP764364PIG
CSB-EP764364PIG
CSB-BP764364PIG
CSB-MP764364PIG
CSB-EP764364PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum ATP synthase subunit beta 1 (atpD1) CSB-YP764365PIG
CSB-EP764365PIG
CSB-BP764365PIG
CSB-MP764365PIG
CSB-EP764365PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP764366PIG
CSB-EP764366PIG
CSB-BP764366PIG
CSB-MP764366PIG
CSB-EP764366PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum ATP-dependent RNA helicase rhlB (rhlB) CSB-YP764367PIG
CSB-EP764367PIG
CSB-BP764367PIG
CSB-MP764367PIG
CSB-EP764367PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP764368PIG
CSB-EP764368PIG
CSB-BP764368PIG
CSB-MP764368PIG
CSB-EP764368PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP764369PIG
CSB-EP764369PIG
CSB-BP764369PIG
CSB-MP764369PIG
CSB-EP764369PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum NADH pyrophosphatase (nudC) CSB-YP764370PIG
CSB-EP764370PIG
CSB-BP764370PIG
CSB-MP764370PIG
CSB-EP764370PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Uncharacterized protein YjeA (yjeA) CSB-YP764371PIG
CSB-EP764371PIG
CSB-BP764371PIG
CSB-MP764371PIG
CSB-EP764371PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 30S ribosomal protein S6 (rpsF) CSB-YP764372PIG
CSB-EP764372PIG
CSB-BP764372PIG
CSB-MP764372PIG
CSB-EP764372PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Siroheme synthase (cysG) CSB-YP764373PIG
CSB-EP764373PIG
CSB-BP764373PIG
CSB-MP764373PIG
CSB-EP764373PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP764374PIG
CSB-EP764374PIG
CSB-BP764374PIG
CSB-MP764374PIG
CSB-EP764374PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UPF0289 protein PBPRA3205 (PBPRA3205) CSB-YP764375PIG
CSB-EP764375PIG
CSB-BP764375PIG
CSB-MP764375PIG
CSB-EP764375PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum S-adenosylmethionine synthase (metK) CSB-YP764376PIG
CSB-EP764376PIG
CSB-BP764376PIG
CSB-MP764376PIG
CSB-EP764376PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ribonuclease 3 (rnc) CSB-YP764377PIG
CSB-EP764377PIG
CSB-BP764377PIG
CSB-MP764377PIG
CSB-EP764377PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ribosome maturation factor RimM (rimM) CSB-YP764378PIG
CSB-EP764378PIG
CSB-BP764378PIG
CSB-MP764378PIG
CSB-EP764378PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Undecaprenyl pyrophosphate synthase (uppS) CSB-YP764379PIG
CSB-EP764379PIG
CSB-BP764379PIG
CSB-MP764379PIG
CSB-EP764379PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP764380PIG
CSB-EP764380PIG
CSB-BP764380PIG
CSB-MP764380PIG
CSB-EP764380PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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