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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Phosphate acyltransferase (plsX) CSB-YP765362BQN
CSB-EP765362BQN
CSB-BP765362BQN
CSB-MP765362BQN
CSB-EP765362BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP765363BQN
CSB-EP765363BQN
CSB-BP765363BQN
CSB-MP765363BQN
CSB-EP765363BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Elongation factor Ts (tsf) CSB-YP765364BQN
CSB-EP765364BQN
CSB-BP765364BQN
CSB-MP765364BQN
CSB-EP765364BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Proline--tRNA ligase 1 (proS1), partial CSB-YP765365BQN
CSB-EP765365BQN
CSB-BP765365BQN
CSB-MP765365BQN
CSB-EP765365BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative competence-damage inducible protein (cinA) CSB-YP765366BQN
CSB-EP765366BQN
CSB-BP765366BQN
CSB-MP765366BQN
CSB-EP765366BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Vpr (vpr) CSB-YP765367HDAK
CSB-EP765367HDAK
CSB-BP765367HDAK
CSB-MP765367HDAK
CSB-EP765367HDAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Hut operon positive regulatory protein (hutP) CSB-YP765368BQN
CSB-EP765368BQN
CSB-BP765368BQN
CSB-MP765368BQN
CSB-EP765368BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein O (sspO) CSB-YP765369BQN
CSB-EP765369BQN
CSB-BP765369BQN
CSB-MP765369BQN
CSB-EP765369BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0178 protein BCE_3095 (BCE_3095) CSB-YP765370BQN
CSB-EP765370BQN
CSB-BP765370BQN
CSB-MP765370BQN
CSB-EP765370BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aliphatic sulfonates import ATP-binding protein SsuB (ssuB) CSB-YP765371BQN
CSB-EP765371BQN
CSB-BP765371BQN
CSB-MP765371BQN
CSB-EP765371BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0421 protein BCE_2776 (BCE_2776), partial CSB-YP765372BQN1
CSB-EP765372BQN1
CSB-BP765372BQN1
CSB-MP765372BQN1
CSB-EP765372BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP765373BQN
CSB-EP765373BQN
CSB-BP765373BQN
CSB-MP765373BQN
CSB-EP765373BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chromosome-anchoring protein racA (racA) CSB-YP765374BQN
CSB-EP765374BQN
CSB-BP765374BQN
CSB-MP765374BQN
CSB-EP765374BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Isoleucine--tRNA ligase 2 (ileS2), partial CSB-YP765375BQN
CSB-EP765375BQN
CSB-BP765375BQN
CSB-MP765375BQN
CSB-EP765375BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Uncharacterized HTH-type transcriptional regulator BCE_2019 (BCE_2019) CSB-YP765376BQN
CSB-EP765376BQN
CSB-BP765376BQN
CSB-MP765376BQN
CSB-EP765376BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable D-serine dehydratase (dsdA) CSB-YP765377BQN
CSB-EP765377BQN
CSB-BP765377BQN
CSB-MP765377BQN
CSB-EP765377BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Demethylmenaquinone methyltransferase (ubiE) CSB-YP765378BQN
CSB-EP765378BQN
CSB-BP765378BQN
CSB-MP765378BQN
CSB-EP765378BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP765379BQN
CSB-EP765379BQN
CSB-BP765379BQN
CSB-MP765379BQN
CSB-EP765379BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Tryptophan synthase alpha chain (trpA) CSB-YP765380BQN
CSB-EP765380BQN
CSB-BP765380BQN
CSB-MP765380BQN
CSB-EP765380BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0477 protein BCE_1348 (BCE_1348) CSB-YP765381BQN
CSB-EP765381BQN
CSB-BP765381BQN
CSB-MP765381BQN
CSB-EP765381BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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