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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Glycerol kinase (glpK), partial CSB-YP765382BQN
CSB-EP765382BQN
CSB-BP765382BQN
CSB-MP765382BQN
CSB-EP765382BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus FMN-dependent NADH-azoreductase 1 (azoR1) CSB-YP765383BQN
CSB-EP765383BQN
CSB-BP765383BQN
CSB-MP765383BQN
CSB-EP765383BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-ribose pyranase (rbsD) CSB-YP765384BQN
CSB-EP765384BQN
CSB-BP765384BQN
CSB-MP765384BQN
CSB-EP765384BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP765385BQN
CSB-EP765385BQN
CSB-BP765385BQN
CSB-MP765385BQN
CSB-EP765385BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus tRNA pseudouridine synthase A 2 (truA2) CSB-YP765386BQN
CSB-EP765386BQN
CSB-BP765386BQN
CSB-MP765386BQN
CSB-EP765386BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Hydroxyethylthiazole kinase (thiM) CSB-YP765387BQN
CSB-EP765387BQN
CSB-BP765387BQN
CSB-MP765387BQN
CSB-EP765387BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP765388BQN
CSB-EP765388BQN
CSB-BP765388BQN
CSB-MP765388BQN
CSB-EP765388BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 10 kDa chaperonin (groS) CSB-YP765389BQN
CSB-EP765389BQN
CSB-BP765389BQN
CSB-MP765389BQN
CSB-EP765389BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protein sprT-like (BCE_0277) CSB-YP765390BQN
CSB-EP765390BQN
CSB-BP765390BQN
CSB-MP765390BQN
CSB-EP765390BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Energy-coupling factor transporter ATP-binding protein EcfA 2 (ecfA2) CSB-YP765391BQN
CSB-EP765391BQN
CSB-BP765391BQN
CSB-MP765391BQN
CSB-EP765391BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S5 (rpsE) CSB-YP765392BQN
CSB-EP765392BQN
CSB-BP765392BQN
CSB-MP765392BQN
CSB-EP765392BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L29 (rpmC) CSB-YP765393BQN
CSB-EP765393BQN
CSB-BP765393BQN
CSB-MP765393BQN
CSB-EP765393BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Elongation factor Tu (tuf) CSB-YP765394BQN
CSB-EP765394BQN
CSB-BP765394BQN
CSB-MP765394BQN
CSB-EP765394BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP765395BQN
CSB-EP765395BQN
CSB-BP765395BQN
CSB-MP765395BQN
CSB-EP765395BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP765396BQN
CSB-EP765396BQN
CSB-BP765396BQN
CSB-MP765396BQN
CSB-EP765396BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Thymidylate kinase (tmk) CSB-YP765397BQN
CSB-EP765397BQN
CSB-BP765397BQN
CSB-MP765397BQN
CSB-EP765397BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor P (efp) CSB-YP765398WAQ
CSB-EP765398WAQ
CSB-BP765398WAQ
CSB-MP765398WAQ
CSB-EP765398WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease 3 (rnc) CSB-YP765399WAQ
CSB-EP765399WAQ
CSB-BP765399WAQ
CSB-MP765399WAQ
CSB-EP765399WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Recombination protein RecR (recR) CSB-YP765400WAQ
CSB-EP765400WAQ
CSB-BP765400WAQ
CSB-MP765400WAQ
CSB-EP765400WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein ApaG (apaG) CSB-YP765401WAQ
CSB-EP765401WAQ
CSB-BP765401WAQ
CSB-MP765401WAQ
CSB-EP765401WAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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