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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP765342BQN
CSB-EP765342BQN
CSB-BP765342BQN
CSB-MP765342BQN
CSB-EP765342BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0354 protein BCE_4835 (BCE_4835) CSB-YP765343BQN
CSB-EP765343BQN
CSB-BP765343BQN
CSB-MP765343BQN
CSB-EP765343BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0756 membrane protein BCE_4726 (BCE_4726), partial CSB-YP765344BQN1
CSB-EP765344BQN1
CSB-BP765344BQN1
CSB-MP765344BQN1
CSB-EP765344BQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L35 (rpmI) CSB-YP765345BQN
CSB-EP765345BQN
CSB-BP765345BQN
CSB-MP765345BQN
CSB-EP765345BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ribonuclease PH (rph) CSB-YP765346BQN
CSB-EP765346BQN
CSB-BP765346BQN
CSB-MP765346BQN
CSB-EP765346BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glutamate-1-semialdehyde 2,1-aminomutase 2 (hemL2) CSB-YP765347BQN
CSB-EP765347BQN
CSB-BP765347BQN
CSB-MP765347BQN
CSB-EP765347BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus GTPase obg (obg) CSB-YP765348BQN
CSB-EP765348BQN
CSB-BP765348BQN
CSB-MP765348BQN
CSB-EP765348BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative Holliday junction resolvase (BCE_4469) CSB-YP765349BQN
CSB-EP765349BQN
CSB-BP765349BQN
CSB-MP765349BQN
CSB-EP765349BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP765350BQN
CSB-EP765350BQN
CSB-BP765350BQN
CSB-MP765350BQN
CSB-EP765350BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chaperone protein DnaJ (dnaJ) CSB-YP765351BQN
CSB-EP765351BQN
CSB-BP765351BQN
CSB-MP765351BQN
CSB-EP765351BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphate import ATP-binding protein PstB (pstB) CSB-YP765352BQN
CSB-EP765352BQN
CSB-BP765352BQN
CSB-MP765352BQN
CSB-EP765352BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-dehydroquinate dehydratase (aroQ) CSB-YP765353BQN
CSB-EP765353BQN
CSB-BP765353BQN
CSB-MP765353BQN
CSB-EP765353BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0403 protein BCE_4227 (BCE_4227) CSB-YP765354BQN
CSB-EP765354BQN
CSB-BP765354BQN
CSB-MP765354BQN
CSB-EP765354BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Acireductone dioxygenase (mtnD) CSB-YP765355BQN
CSB-EP765355BQN
CSB-BP765355BQN
CSB-MP765355BQN
CSB-EP765355BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Protoheme IX farnesyltransferase (ctaB), partial CSB-YP765356BQN
CSB-EP765356BQN
CSB-BP765356BQN
CSB-MP765356BQN
CSB-EP765356BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0348 protein BCE_3971 (BCE_3971) CSB-YP765357BQN
CSB-EP765357BQN
CSB-BP765357BQN
CSB-MP765357BQN
CSB-EP765357BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP765358BQN
CSB-EP765358BQN
CSB-BP765358BQN
CSB-MP765358BQN
CSB-EP765358BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UDP-N-acetylenolpyruvoylglucosamine reductase 1 (murB1) CSB-YP765359BQN
CSB-EP765359BQN
CSB-BP765359BQN
CSB-MP765359BQN
CSB-EP765359BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydroorotase (pyrC) CSB-YP765360BQN
CSB-EP765360BQN
CSB-BP765360BQN
CSB-MP765360BQN
CSB-EP765360BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0296 protein BCE_3915 (BCE_3915) CSB-YP765361BQN
CSB-EP765361BQN
CSB-BP765361BQN
CSB-MP765361BQN
CSB-EP765361BQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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