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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP765462MLA
CSB-EP765462MLA
CSB-BP765462MLA
CSB-MP765462MLA
CSB-EP765462MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP765463MLA
CSB-EP765463MLA
CSB-BP765463MLA
CSB-MP765463MLA
CSB-EP765463MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S15 (rpsO) CSB-YP765464MLA
CSB-EP765464MLA
CSB-BP765464MLA
CSB-MP765464MLA
CSB-EP765464MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Diaminopimelate epimerase (dapF) CSB-YP765465MLA
CSB-EP765465MLA
CSB-BP765465MLA
CSB-MP765465MLA
CSB-EP765465MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP765466MLA
CSB-EP765466MLA
CSB-BP765466MLA
CSB-MP765466MLA
CSB-EP765466MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant FO synthase (fbiC), partial CSB-YP765467MLA
CSB-EP765467MLA
CSB-BP765467MLA
CSB-MP765467MLA
CSB-EP765467MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 1 (prfA) CSB-YP765468MLA
CSB-EP765468MLA
CSB-BP765468MLA
CSB-MP765468MLA
CSB-EP765468MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP765469MLA
CSB-EP765469MLA
CSB-BP765469MLA
CSB-MP765469MLA
CSB-EP765469MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease proteolytic subunit 1 (clpP1) CSB-YP765470MLA
CSB-EP765470MLA
CSB-BP765470MLA
CSB-MP765470MLA
CSB-EP765470MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L27 (rpmA) CSB-YP765471MLA
CSB-EP765471MLA
CSB-BP765471MLA
CSB-MP765471MLA
CSB-EP765471MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfate/thiosulfate import ATP-binding protein CysA (cysA) CSB-YP765472MLA
CSB-EP765472MLA
CSB-BP765472MLA
CSB-MP765472MLA
CSB-EP765472MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTPase Era (era) CSB-YP765473MLA
CSB-EP765473MLA
CSB-BP765473MLA
CSB-MP765473MLA
CSB-EP765473MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfite reductase [ferredoxin] 1 (sir1), partial CSB-YP765474MLA
CSB-EP765474MLA
CSB-BP765474MLA
CSB-MP765474MLA
CSB-EP765474MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP765475MLA
CSB-EP765475MLA
CSB-BP765475MLA
CSB-MP765475MLA
CSB-EP765475MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP765476MLA
CSB-EP765476MLA
CSB-BP765476MLA
CSB-MP765476MLA
CSB-EP765476MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteasome subunit alpha (prcA) CSB-YP765477MLA
CSB-EP765477MLA
CSB-BP765477MLA
CSB-MP765477MLA
CSB-EP765477MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkyl hydroperoxide reductase AhpD (ahpD) CSB-YP765478MLA
CSB-EP765478MLA
CSB-BP765478MLA
CSB-MP765478MLA
CSB-EP765478MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein translocase subunit SecA 2 (secA2), partial CSB-YP765479MLA
CSB-EP765479MLA
CSB-BP765479MLA
CSB-MP765479MLA
CSB-EP765479MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dephospho-CoA kinase (coaE) CSB-YP765480MLA
CSB-EP765480MLA
CSB-BP765480MLA
CSB-MP765480MLA
CSB-EP765480MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferrochelatase (hemH) CSB-YP765481MLA
CSB-EP765481MLA
CSB-BP765481MLA
CSB-MP765481MLA
CSB-EP765481MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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