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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus laevis Phenylalanine--tRNA ligase alpha subunit B (farsa-b) CSB-YP766463XBE
CSB-EP766463XBE
CSB-BP766463XBE
CSB-MP766463XBE
CSB-EP766463XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Exportin-2 (cse1l), partial CSB-YP766464DIL
CSB-EP766464DIL
CSB-BP766464DIL
CSB-MP766464DIL
CSB-EP766464DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Raftlin (RFTN1), partial CSB-YP766465CH
CSB-EP766465CH
CSB-BP766465CH
CSB-MP766465CH
CSB-EP766465CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Small ubiquitin-related modifier 1 (sumo1) CSB-YP766466DIL
CSB-EP766466DIL
CSB-BP766466DIL
CSB-MP766466DIL
CSB-EP766466DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Serine/threonine-protein kinase 40 (STK40) CSB-YP766467CH
CSB-EP766467CH
CSB-BP766467CH
CSB-MP766467CH
CSB-EP766467CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cytosolic phospholipase A2 (pla2g4a), partial CSB-YP766468XBE
CSB-EP766468XBE
CSB-BP766468XBE
CSB-MP766468XBE
CSB-EP766468XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Coiled-coil domain-containing protein 164 (ccdc164), partial CSB-YP766469XBE
CSB-EP766469XBE
CSB-BP766469XBE
CSB-MP766469XBE
CSB-EP766469XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Peptidase inhibitor 15-A (pi15a) CSB-YP766470DIL
CSB-EP766470DIL
CSB-BP766470DIL
CSB-MP766470DIL
CSB-EP766470DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus flaviceps flaviceps Phospholipase A2, beta bungarotoxin A1 chain CSB-YP766471BXM
CSB-EP766471BXM
CSB-BP766471BXM
CSB-MP766471BXM
CSB-EP766471BXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Hepcidin-2 (hamp2) CSB-YP766472DIL
CSB-EP766472DIL
CSB-BP766472DIL
CSB-MP766472DIL
CSB-EP766472DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio DET1- and DDB1-associated protein 1 (dda1) CSB-YP766473DIL
CSB-EP766473DIL
CSB-BP766473DIL
CSB-MP766473DIL
CSB-EP766473DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Endoplasmic reticulum-Golgi intermediate compartment protein 2 (ergic2), partial CSB-YP766474DIL1
CSB-EP766474DIL1
CSB-BP766474DIL1
CSB-MP766474DIL1
CSB-EP766474DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Interleukin-17 receptor D (IL17RD), partial CSB-YP766475CH
CSB-EP766475CH
CSB-BP766475CH
CSB-MP766475CH
CSB-EP766475CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus flaviceps flaviceps Phospholipase A2-1 CSB-YP766476BXM
CSB-EP766476BXM
CSB-BP766476BXM
CSB-MP766476BXM
CSB-EP766476BXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Netrin receptor UNC5C (UNC5C), partial CSB-YP766477CH
CSB-EP766477CH
CSB-BP766477CH
CSB-MP766477CH
CSB-EP766477CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein CDV3 homolog (cdv3) CSB-YP766478DIL
CSB-EP766478DIL
CSB-BP766478DIL
CSB-MP766478DIL
CSB-EP766478DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein CutA homolog (cuta) CSB-YP766479DIL
CSB-EP766479DIL
CSB-BP766479DIL
CSB-MP766479DIL
CSB-EP766479DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Lariat debranching enzyme (dbr1), partial CSB-YP766480DIL
CSB-EP766480DIL
CSB-BP766480DIL
CSB-MP766480DIL
CSB-EP766480DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis RNA-binding protein 38 (rbm38) CSB-YP766481XBE
CSB-EP766481XBE
CSB-BP766481XBE
CSB-MP766481XBE
CSB-EP766481XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L15 (rpl15) CSB-YP766482PIAC
CSB-EP766482PIAC
CSB-BP766482PIAC
CSB-MP766482PIAC
CSB-EP766482PIAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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