Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dictyostelium discoideum Dolichol-phosphate mannosyltransferase subunit 3 (dpm3), partial CSB-YP765742DKK1
CSB-EP765742DKK1
CSB-BP765742DKK1
CSB-MP765742DKK1
CSB-EP765742DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Importin subunit alpha-B (DDB_G0272318), partial CSB-YP765743DKK
CSB-EP765743DKK
CSB-BP765743DKK
CSB-MP765743DKK
CSB-EP765743DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variola virus Ankyrin repeat protein G3R (G3R), partial CSB-YP765744VAR
CSB-EP765744VAR
CSB-BP765744VAR
CSB-MP765744VAR
CSB-EP765744VAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype C subtype ayr Capsid protein (C) CSB-YP765745HEG
CSB-EP765745HEG
CSB-BP765745HEG
CSB-MP765745HEG
CSB-EP765745HEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Deoxyuridine 5'-triphosphate nucleotidohydrolase (DUT) CSB-YP765746VEE
CSB-EP765746VEE
CSB-BP765746VEE
CSB-MP765746VEE
CSB-EP765746VEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 1 (M) CSB-YP765747IIA
CSB-EP765747IIA
CSB-BP765747IIA
CSB-MP765747IIA
CSB-EP765747IIA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 8.8 kDa protein in frd-Gp32 intergenic region (frd.3) CSB-YP765748BEAA
CSB-EP765748BEAA
CSB-BP765748BEAA
CSB-MP765748BEAA
CSB-EP765748BEAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid glycoprotein VP7 CSB-YP765749RGQ
CSB-EP765749RGQ
CSB-BP765749RGQ
CSB-MP765749RGQ
CSB-EP765749RGQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus DNA-directed RNA polymerase 18 kDa subunit (RPO18) CSB-YP765750VEE
CSB-EP765750VEE
CSB-BP765750VEE
CSB-MP765750VEE
CSB-EP765750VEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group O Gag-Pol polyprotein (gag-pol), partial CSB-YP765751HLS
CSB-EP765751HLS
CSB-BP765751HLS
CSB-MP765751HLS
CSB-EP765751HLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Camelpox virus Profilin (CMP158R) CSB-YP765752CBR
CSB-EP765752CBR
CSB-BP765752CBR
CSB-MP765752CBR
CSB-EP765752CBR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Camelpox virus Glutaredoxin-2 (CMP78L) CSB-YP765753CBR
CSB-EP765753CBR
CSB-BP765753CBR
CSB-MP765753CBR
CSB-EP765753CBR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella phage 7888 Shiga toxin subunit B (stxB) CSB-YP765754SGAM
CSB-EP765754SGAM
CSB-BP765754SGAM
CSB-MP765754SGAM
CSB-EP765754SGAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cowpox virus Profilin (CPXV179) CSB-YP765755CRG
CSB-EP765755CRG
CSB-BP765755CRG
CSB-MP765755CRG
CSB-EP765755CRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 2 (M), partial CSB-YP765756IES1
CSB-EP765756IES1
CSB-BP765756IES1
CSB-MP765756IES1
CSB-EP765756IES1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gallid herpesvirus 2 Envelope glycoprotein E (MDV096), partial CSB-YP765757GAN1
CSB-EP765757GAN1
CSB-BP765757GAN1
CSB-MP765757GAN1
CSB-EP765757GAN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gallid herpesvirus 2 Uncharacterized gene 69 protein (MDV069) CSB-YP765758GAN
CSB-EP765758GAN
CSB-BP765758GAN
CSB-MP765758GAN
CSB-EP765758GAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gallid herpesvirus 2 Ribonucleoside-diphosphate reductase large subunit (MDV052), partial CSB-YP765759GAN
CSB-EP765759GAN
CSB-BP765759GAN
CSB-MP765759GAN
CSB-EP765759GAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gallid herpesvirus 2 Virulence factor MDV010 (MDV010), partial CSB-YP765760GAN
CSB-EP765760GAN
CSB-BP765760GAN
CSB-MP765760GAN
CSB-EP765760GAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6B Protein U90 (U90), partial CSB-YP765761HKA
CSB-EP765761HKA
CSB-BP765761HKA
CSB-MP765761HKA
CSB-EP765761HKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>