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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO (trmFO) CSB-YP767544BQE
CSB-EP767544BQE
CSB-BP767544BQE
CSB-MP767544BQE
CSB-EP767544BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proline--tRNA ligase 1 (proS1), partial CSB-YP767545BQE
CSB-EP767545BQE
CSB-BP767545BQE
CSB-MP767545BQE
CSB-EP767545BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative competence-damage inducible protein (cinA) CSB-YP767546BQE
CSB-EP767546BQE
CSB-BP767546BQE
CSB-MP767546BQE
CSB-EP767546BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein hfq (hfq) CSB-YP767547BQE
CSB-EP767547BQE
CSB-BP767547BQE
CSB-MP767547BQE
CSB-EP767547BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP767548BQE1
CSB-EP767548BQE1
CSB-BP767548BQE1
CSB-MP767548BQE1
CSB-EP767548BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP767549BQE
CSB-EP767549BQE
CSB-BP767549BQE
CSB-MP767549BQE
CSB-EP767549BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Diaminopimelate epimerase (dapF) CSB-YP767550BQE
CSB-EP767550BQE
CSB-BP767550BQE
CSB-MP767550BQE
CSB-EP767550BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hut operon positive regulatory protein (hutP) CSB-YP767551BQE
CSB-EP767551BQE
CSB-BP767551BQE
CSB-MP767551BQE
CSB-EP767551BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epoxyqueuosine reductase (queG) CSB-YP767552BQE
CSB-EP767552BQE
CSB-BP767552BQE
CSB-MP767552BQE
CSB-EP767552BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small, acid-soluble spore protein K (sspK) CSB-YP767553BQE
CSB-EP767553BQE
CSB-BP767553BQE
CSB-MP767553BQE
CSB-EP767553BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0435 protein BA_0406/GBAA_0406/BAS0392 (BA_0406, GBAA_0406, BAS0392) CSB-YP767554BQE
CSB-EP767554BQE
CSB-BP767554BQE
CSB-MP767554BQE
CSB-EP767554BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP767555BQE
CSB-EP767555BQE
CSB-BP767555BQE
CSB-MP767555BQE
CSB-EP767555BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP767556BQE
CSB-EP767556BQE
CSB-BP767556BQE
CSB-MP767556BQE
CSB-EP767556BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP767557SNN
CSB-EP767557SNN
CSB-BP767557SNN
CSB-MP767557SNN
CSB-EP767557SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP767558NHH
CSB-EP767558NHH
CSB-BP767558NHH
CSB-MP767558NHH
CSB-EP767558NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural glycoprotein 4, partial CSB-YP767559RGN
CSB-EP767559RGN
CSB-BP767559RGN
CSB-MP767559RGN
CSB-EP767559RGN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 3 CSB-YP767560RNW
CSB-EP767560RNW
CSB-BP767560RNW
CSB-MP767560RNW
CSB-EP767560RNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human rhinovirus 3 Genome polyprotein CSB-YP767561HDAW
CSB-EP767561HDAW
CSB-BP767561HDAW
CSB-MP767561HDAW
CSB-EP767561HDAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Apolipoprotein N-acyltransferase (lnt), partial CSB-YP767562DXP1
CSB-EP767562DXP1
CSB-BP767562DXP1
CSB-MP767562DXP1
CSB-EP767562DXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP767563TIW
CSB-EP767563TIW
CSB-BP767563TIW
CSB-MP767563TIW
CSB-EP767563TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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