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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii Putative lipase ATG15 (ATG15), partial CSB-YP765682DOT
CSB-EP765682DOT
CSB-BP765682DOT
CSB-MP765682DOT
CSB-EP765682DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii V-type proton ATPase subunit e (VMA9), partial CSB-YP765683DOT1
CSB-EP765683DOT1
CSB-BP765683DOT1
CSB-MP765683DOT1
CSB-EP765683DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mediator of RNA polymerase II transcription subunit 6 (MED6) CSB-YP765684DOT
CSB-EP765684DOT
CSB-BP765684DOT
CSB-MP765684DOT
CSB-EP765684DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii HDA1 complex subunit 2 (HDA2), partial CSB-YP765685DOT
CSB-EP765685DOT
CSB-BP765685DOT
CSB-MP765685DOT
CSB-EP765685DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Autophagy-related protein 18 (ATG18), partial CSB-YP765686DOT
CSB-EP765686DOT
CSB-BP765686DOT
CSB-MP765686DOT
CSB-EP765686DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Maintenance of mitochondrial morphology protein 1 (MMM1), partial CSB-YP765687DOT
CSB-EP765687DOT
CSB-BP765687DOT
CSB-MP765687DOT
CSB-EP765687DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 3-dehydroquinate synthase (aroB) CSB-YP765688LEV
CSB-EP765688LEV
CSB-BP765688LEV
CSB-MP765688LEV
CSB-EP765688LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ribosomal protein VAR1, mitochondrial (VAR1) CSB-YP765689DOT
CSB-EP765689DOT
CSB-BP765689DOT
CSB-MP765689DOT
CSB-EP765689DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Auxin-responsive protein IAA12 (IAA12) CSB-YP765690OFG
CSB-EP765690OFG
CSB-BP765690OFG
CSB-MP765690OFG
CSB-EP765690OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein argonaute 7 (AGO7), partial CSB-YP765691OFG
CSB-EP765691OFG
CSB-BP765691OFG
CSB-MP765691OFG
CSB-EP765691OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein SHORT-ROOT 2 (SHR2), partial CSB-YP765692OFG
CSB-EP765692OFG
CSB-BP765692OFG
CSB-MP765692OFG
CSB-EP765692OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Outer envelope pore protein 24, chloroplastic (OEP24), partial CSB-YP765693OFG
CSB-EP765693OFG
CSB-BP765693OFG
CSB-MP765693OFG
CSB-EP765693OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum HssA/B-like protein 11 (hssl11) CSB-YP765694DKK
CSB-EP765694DKK
CSB-BP765694DKK
CSB-MP765694DKK
CSB-EP765694DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0276161 (DDB_G0276161) CSB-YP765695DKK
CSB-EP765695DKK
CSB-BP765695DKK
CSB-MP765695DKK
CSB-EP765695DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Vesicle-fusing ATPase (nsfA), partial CSB-YP765696DKK
CSB-EP765696DKK
CSB-BP765696DKK
CSB-MP765696DKK
CSB-EP765696DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Transmembrane protein 184 homolog DDB_G0276041 (tmem184B), partial CSB-YP765697DKK1
CSB-EP765697DKK1
CSB-BP765697DKK1
CSB-MP765697DKK1
CSB-EP765697DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Peroxisome biogenesis factor 2 (pex2), partial CSB-YP765698DKK1
CSB-EP765698DKK1
CSB-BP765698DKK1
CSB-MP765698DKK1
CSB-EP765698DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum GATA zinc finger domain-containing protein 3 (gtaC), partial CSB-YP765699DKK
CSB-EP765699DKK
CSB-BP765699DKK
CSB-MP765699DKK
CSB-EP765699DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Glucose and ribitol dehydrogenase homolog (Os05g0140800, LOC_Os05g04870) CSB-YP765700OFG
CSB-EP765700OFG
CSB-BP765700OFG
CSB-MP765700OFG
CSB-EP765700OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable histone H2A.6 (Os05g0113900, LOC_Os05g02300) CSB-YP765701OFG
CSB-EP765701OFG
CSB-BP765701OFG
CSB-MP765701OFG
CSB-EP765701OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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