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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP766202PIJ
CSB-EP766202PIJ
CSB-BP766202PIJ
CSB-MP766202PIJ
CSB-EP766202PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP766203PIJ
CSB-EP766203PIJ
CSB-BP766203PIJ
CSB-MP766203PIJ
CSB-EP766203PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP766204PIJ
CSB-EP766204PIJ
CSB-BP766204PIJ
CSB-MP766204PIJ
CSB-EP766204PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP766205PIJ
CSB-EP766205PIJ
CSB-BP766205PIJ
CSB-MP766205PIJ
CSB-EP766205PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Cysteine desulfuration protein sufE (sufE) CSB-YP766206PIJ
CSB-EP766206PIJ
CSB-BP766206PIJ
CSB-MP766206PIJ
CSB-EP766206PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Tyrosine--tRNA ligase 1 (tyrS1) CSB-YP766207PIJ
CSB-EP766207PIJ
CSB-BP766207PIJ
CSB-MP766207PIJ
CSB-EP766207PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii D-amino acid dehydrogenase small subunit (dadA) CSB-YP766208PIJ
CSB-EP766208PIJ
CSB-BP766208PIJ
CSB-MP766208PIJ
CSB-EP766208PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Tryptophan synthase alpha chain (trpA) CSB-YP766209PIJ
CSB-EP766209PIJ
CSB-BP766209PIJ
CSB-MP766209PIJ
CSB-EP766209PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Electron transport complex protein RnfG (rnfG), partial CSB-YP766210PIJ1
CSB-EP766210PIJ1
CSB-BP766210PIJ1
CSB-MP766210PIJ1
CSB-EP766210PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD (+) reductase component (hcaD) CSB-YP766211PIJ
CSB-EP766211PIJ
CSB-BP766211PIJ
CSB-MP766211PIJ
CSB-EP766211PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Urease accessory protein UreG (ureG) CSB-YP766212PIJ
CSB-EP766212PIJ
CSB-BP766212PIJ
CSB-MP766212PIJ
CSB-EP766212PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UPF0745 protein plu2139 (plu2139) CSB-YP766213PIJ
CSB-EP766213PIJ
CSB-BP766213PIJ
CSB-MP766213PIJ
CSB-EP766213PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP766214PIJ
CSB-EP766214PIJ
CSB-BP766214PIJ
CSB-MP766214PIJ
CSB-EP766214PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Acyl transferase (luxD) CSB-YP766215PIJ
CSB-EP766215PIJ
CSB-BP766215PIJ
CSB-MP766215PIJ
CSB-EP766215PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Aquaporin Z (aqpZ), partial CSB-YP766216PIJ1
CSB-EP766216PIJ1
CSB-BP766216PIJ1
CSB-MP766216PIJ1
CSB-EP766216PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Flagellar L-ring protein (flgH) CSB-YP766217PIJ
CSB-EP766217PIJ
CSB-BP766217PIJ
CSB-MP766217PIJ
CSB-EP766217PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Methylglyoxal synthase (mgsA) CSB-YP766218PIJ
CSB-EP766218PIJ
CSB-BP766218PIJ
CSB-MP766218PIJ
CSB-EP766218PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Chromosome partition protein mukE (mukE) CSB-YP766219PIJ
CSB-EP766219PIJ
CSB-BP766219PIJ
CSB-MP766219PIJ
CSB-EP766219PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP766220PIJ
CSB-EP766220PIJ
CSB-BP766220PIJ
CSB-MP766220PIJ
CSB-EP766220PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Outer-membrane lipoprotein carrier protein (lolA) CSB-YP766221PIJ
CSB-EP766221PIJ
CSB-BP766221PIJ
CSB-MP766221PIJ
CSB-EP766221PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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