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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human AT-hook-containing transcription factor (AKNA), partial CSB-YP767203HU
CSB-EP767203HU
CSB-BP767203HU
CSB-MP767203HU
CSB-EP767203HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Vomeronasal type-1 receptor 4 (VN1R4), partial CSB-YP767204HU1
CSB-EP767204HU1
CSB-BP767204HU1
CSB-MP767204HU1
CSB-EP767204HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cytoplasmic polyadenylation element-binding protein 2 (CPEB2) CSB-YP767205HU
CSB-EP767205HU
CSB-BP767205HU
CSB-MP767205HU
CSB-EP767205HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Probable methyltransferase BCDIN3D (BCDIN3D) CSB-YP767206HU
CSB-EP767206HU
CSB-BP767206HU
CSB-MP767206HU
CSB-EP767206HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ankyrin repeat and sterile alpha motif domain-containing protein 1B (ANKS1B), partial CSB-YP767207HU
CSB-EP767207HU
CSB-BP767207HU
CSB-MP767207HU
CSB-EP767207HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein prenyltransferase alpha subunit repeat-containing protein 1 (PTAR1) CSB-YP767208HU
CSB-EP767208HU
CSB-BP767208HU
CSB-MP767208HU
CSB-EP767208HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human F-box only protein 33 (FBXO33) CSB-YP767209HU
CSB-EP767209HU
CSB-BP767209HU
CSB-MP767209HU
CSB-EP767209HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human tRNA-specific adenosine deaminase 2 (ADAT2) CSB-YP767210HU
CSB-EP767210HU
CSB-BP767210HU
CSB-MP767210HU
CSB-EP767210HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Stimulated by retinoic acid gene 8 protein homolog (STRA8) CSB-YP767212HU
CSB-EP767212HU
CSB-BP767212HU
CSB-MP767212HU
CSB-EP767212HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Complexin-4 (CPLX4) CSB-YP767213HU
CSB-EP767213HU
CSB-BP767213HU
CSB-MP767213HU
CSB-EP767213HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 39S ribosomal protein L10, mitochondrial (MRPL10) CSB-YP767214HU
CSB-EP767214HU
CSB-BP767214HU
CSB-MP767214HU
CSB-EP767214HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Probable G-protein coupled receptor 144 (GPR144), partial CSB-YP767215HU
CSB-EP767215HU
CSB-BP767215HU
CSB-MP767215HU
CSB-EP767215HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea DNA ligase 4 (LIG4), partial CSB-YP767216DWP
CSB-EP767216DWP
CSB-BP767216DWP
CSB-MP767216DWP
CSB-EP767216DWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colletotrichum gloeosporioides Glycerol-3-phosphate dehydrogenase [NAD (+)] CSB-YP767217DVE
CSB-EP767217DVE
CSB-BP767217DVE
CSB-MP767217DVE
CSB-EP767217DVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized ABC transporter ATP-binding protein C20G4.01 (SPAC20G4.01) CSB-YP767218SXV
CSB-EP767218SXV
CSB-BP767218SXV
CSB-MP767218SXV
CSB-EP767218SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized oxidoreductase C663.08c (SPCC663.08c) CSB-YP767219SXV
CSB-EP767219SXV
CSB-BP767219SXV
CSB-MP767219SXV
CSB-EP767219SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pichia angusta Glycerol-3-phosphate dehydrogenase [NAD (+)] (GPD) CSB-YP767220EVQ
CSB-EP767220EVQ
CSB-BP767220EVQ
CSB-MP767220EVQ
CSB-EP767220EVQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Tyrosine recombinase XerC (xerC) CSB-YP767221SLD
CSB-EP767221SLD
CSB-BP767221SLD
CSB-MP767221SLD
CSB-EP767221SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Segregation and condensation protein A (scpA) CSB-YP767222FMR
CSB-EP767222FMR
CSB-BP767222FMR
CSB-MP767222FMR
CSB-EP767222FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine recombinase XerC (xerC) CSB-YP767223SZB
CSB-EP767223SZB
CSB-BP767223SZB
CSB-MP767223SZB
CSB-EP767223SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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