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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Helicobacter hepaticus GTPase Der (der) CSB-YP766803HCO
CSB-EP766803HCO
CSB-BP766803HCO
CSB-MP766803HCO
CSB-EP766803HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Serine--tRNA ligase (serS) CSB-YP766804HCO
CSB-EP766804HCO
CSB-BP766804HCO
CSB-MP766804HCO
CSB-EP766804HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 50S ribosomal protein L17 (rplQ) CSB-YP766805HCO
CSB-EP766805HCO
CSB-BP766805HCO
CSB-MP766805HCO
CSB-EP766805HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 50S ribosomal protein L6 (rplF) CSB-YP766806HCO
CSB-EP766806HCO
CSB-BP766806HCO
CSB-MP766806HCO
CSB-EP766806HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 30S ribosomal protein S3 (rpsC) CSB-YP766807HCO
CSB-EP766807HCO
CSB-BP766807HCO
CSB-MP766807HCO
CSB-EP766807HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Probable cytosol aminopeptidase (pepA) CSB-YP766808HCO
CSB-EP766808HCO
CSB-BP766808HCO
CSB-MP766808HCO
CSB-EP766808HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Probable GTP-binding protein EngB (engB) CSB-YP766809HCO
CSB-EP766809HCO
CSB-BP766809HCO
CSB-MP766809HCO
CSB-EP766809HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP766810HCO
CSB-EP766810HCO
CSB-BP766810HCO
CSB-MP766810HCO
CSB-EP766810HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Ketol-acid reductoisomerase (ilvC) CSB-YP766811HCO
CSB-EP766811HCO
CSB-BP766811HCO
CSB-MP766811HCO
CSB-EP766811HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus 3-isopropylmalate dehydrogenase (leuB) CSB-YP766812HCO
CSB-EP766812HCO
CSB-BP766812HCO
CSB-MP766812HCO
CSB-EP766812HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP766813HCO
CSB-EP766813HCO
CSB-BP766813HCO
CSB-MP766813HCO
CSB-EP766813HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus UPF0763 protein HH_0976 (HH_0976) CSB-YP766814HCO
CSB-EP766814HCO
CSB-BP766814HCO
CSB-MP766814HCO
CSB-EP766814HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Flagellar assembly factor FliW 2 (fliW2) CSB-YP766815HCO
CSB-EP766815HCO
CSB-BP766815HCO
CSB-MP766815HCO
CSB-EP766815HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Alanine--tRNA ligase (alaS), partial CSB-YP766816HCO
CSB-EP766816HCO
CSB-BP766816HCO
CSB-MP766816HCO
CSB-EP766816HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Ribosomal protein L11 methyltransferase (prmA) CSB-YP766817HCO
CSB-EP766817HCO
CSB-BP766817HCO
CSB-MP766817HCO
CSB-EP766817HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP766818HCO
CSB-EP766818HCO
CSB-BP766818HCO
CSB-MP766818HCO
CSB-EP766818HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Acyl carrier protein (acpP) CSB-YP766819HCO
CSB-EP766819HCO
CSB-BP766819HCO
CSB-MP766819HCO
CSB-EP766819HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Chorismate synthase (aroC) CSB-YP766820HCO
CSB-EP766820HCO
CSB-BP766820HCO
CSB-MP766820HCO
CSB-EP766820HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Protein grpE (grpE) CSB-YP766821HCO
CSB-EP766821HCO
CSB-BP766821HCO
CSB-MP766821HCO
CSB-EP766821HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP766822HCO
CSB-EP766822HCO
CSB-BP766822HCO
CSB-MP766822HCO
CSB-EP766822HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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