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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neurospora crassa 40S ribosomal protein S16 (rps-16) CSB-YP766443NHA
CSB-EP766443NHA
CSB-BP766443NHA
CSB-MP766443NHA
CSB-EP766443NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa RNA polymerase II subunit A C-terminal domain phosphatase ssu-72 (ssu-72) CSB-YP766444NHA
CSB-EP766444NHA
CSB-BP766444NHA
CSB-MP766444NHA
CSB-EP766444NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Golgi apparatus membrane protein tvp-23 (tvp-23), partial CSB-YP766445NHA1
CSB-EP766445NHA1
CSB-BP766445NHA1
CSB-MP766445NHA1
CSB-EP766445NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa High osmolarity signaling protein sho1 (sho1), partial CSB-YP766446NHA1
CSB-EP766446NHA1
CSB-BP766446NHA1
CSB-MP766446NHA1
CSB-EP766446NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Elongation factor G, mitochondrial (mef-1), partial CSB-YP766447NHA
CSB-EP766447NHA
CSB-BP766447NHA
CSB-MP766447NHA
CSB-EP766447NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Chromosome segregation in meiosis protein 3 (csm-3) CSB-YP766448NHA
CSB-EP766448NHA
CSB-BP766448NHA
CSB-MP766448NHA
CSB-EP766448NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein efr-3 (efr-3), partial CSB-YP766449NHA
CSB-EP766449NHA
CSB-BP766449NHA
CSB-MP766449NHA
CSB-EP766449NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Pre-mRNA-splicing factor cwc-25 (cwc-25) CSB-YP766450NHA
CSB-EP766450NHA
CSB-BP766450NHA
CSB-MP766450NHA
CSB-EP766450NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eptatretus burgeri Globin-F1 CSB-YP766451EFF
CSB-EP766451EFF
CSB-BP766451EFF
CSB-MP766451EFF
CSB-EP766451EFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meleagris gallopavo Delta-1 crystallin (ASL1) CSB-YP766452MQV
CSB-EP766452MQV
CSB-BP766452MQV
CSB-MP766452MQV
CSB-EP766452MQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Elastase-1 CSB-YP766453SWI
CSB-BP766453SWI
CSB-MP766453SWI
CSB-EP766453SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Major facilitator superfamily domain-containing protein 4-A (mfsd4a), partial CSB-YP766454DIL
CSB-EP766454DIL
CSB-BP766454DIL
CSB-MP766454DIL
CSB-EP766454DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Pre-mRNA-splicing regulator WTAP (wtap) CSB-YP766455DIL
CSB-EP766455DIL
CSB-BP766455DIL
CSB-MP766455DIL
CSB-EP766455DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein YIPF5 (yipf5), partial CSB-YP766456XBE1
CSB-EP766456XBE1
CSB-BP766456XBE1
CSB-MP766456XBE1
CSB-EP766456XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Solute carrier family 25 member 39 (slc25a39), partial CSB-YP766457DIL1
CSB-EP766457DIL1
CSB-BP766457DIL1
CSB-MP766457DIL1
CSB-EP766457DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio GPI mannosyltransferase 3 (pigb), partial CSB-YP766458DIL
CSB-EP766458DIL
CSB-BP766458DIL
CSB-MP766458DIL
CSB-EP766458DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Keratin, type I cytoskeletal 18-B (krt18-b) CSB-YP766459XBE
CSB-EP766459XBE
CSB-BP766459XBE
CSB-MP766459XBE
CSB-EP766459XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Autophagy-related protein 13 (atg13), partial CSB-YP766460DIL
CSB-EP766460DIL
CSB-BP766460DIL
CSB-MP766460DIL
CSB-EP766460DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Queuine tRNA-ribosyltransferase (qtrt1) CSB-YP766461DIL
CSB-EP766461DIL
CSB-BP766461DIL
CSB-MP766461DIL
CSB-EP766461DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Charged multivesicular body protein 2b-A (chmp2b-a) CSB-YP766462XBE
CSB-EP766462XBE
CSB-BP766462XBE
CSB-MP766462XBE
CSB-EP766462XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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