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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Phosphoglucomutase-2 (Pgm2) CSB-YP766523MO
CSB-EP766523MO
CSB-BP766523MO
CSB-MP766523MO
CSB-EP766523MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse CUGBP Elav-like family member 4 (Celf4) CSB-YP766524MO
CSB-EP766524MO
CSB-BP766524MO
CSB-MP766524MO
CSB-EP766524MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Probable G-protein coupled receptor 125 (Gpr125), partial CSB-YP766525MO
CSB-EP766525MO
CSB-BP766525MO
CSB-MP766525MO
CSB-EP766525MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein phosphatase 1 regulatory subunit 14D (Ppp1r14d) CSB-YP766526MO
CSB-EP766526MO
CSB-BP766526MO
CSB-MP766526MO
CSB-EP766526MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavohemoprotein (hmp) CSB-YP766527BIK
CSB-EP766527BIK
CSB-BP766527BIK
CSB-MP766527BIK
CSB-EP766527BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Tryptophan synthase beta chain (trpB) CSB-YP766528SSL
CSB-EP766528SSL
CSB-BP766528SSL
CSB-MP766528SSL
CSB-EP766528SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP766529SSL
CSB-EP766529SSL
CSB-BP766529SSL
CSB-MP766529SSL
CSB-EP766529SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal protein L11 methyltransferase (prmA) CSB-YP766530SSL
CSB-EP766530SSL
CSB-BP766530SSL
CSB-MP766530SSL
CSB-EP766530SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP766531RDR
CSB-EP766531RDR
CSB-BP766531RDR
CSB-MP766531RDR
CSB-EP766531RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica tRNA pseudouridine synthase A (truA) CSB-YP766532RDR
CSB-EP766532RDR
CSB-BP766532RDR
CSB-MP766532RDR
CSB-EP766532RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 30S ribosomal protein S19 (rpsS) CSB-YP766533EYQ
CSB-EP766533EYQ
CSB-BP766533EYQ
CSB-MP766533EYQ
CSB-EP766533EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Ribosomal protein L11 methyltransferase (prmA) CSB-YP766534EYQ
CSB-EP766534EYQ
CSB-BP766534EYQ
CSB-MP766534EYQ
CSB-EP766534EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 30S ribosomal protein S21 (rpsU) CSB-YP766535EYQ
CSB-EP766535EYQ
CSB-BP766535EYQ
CSB-MP766535EYQ
CSB-EP766535EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Methionyl-tRNA formyltransferase (fmt) CSB-YP766536EYQ
CSB-EP766536EYQ
CSB-BP766536EYQ
CSB-MP766536EYQ
CSB-EP766536EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris NAD (P)H-quinone oxidoreductase subunit 3 (ndhC), partial CSB-YP766537EYQ1
CSB-EP766537EYQ1
CSB-BP766537EYQ1
CSB-MP766537EYQ1
CSB-EP766537EYQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Tryptophan synthase beta chain (trpB) CSB-YP766538EYO
CSB-EP766538EYO
CSB-BP766538EYO
CSB-MP766538EYO
CSB-EP766538EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L14 (rplN) CSB-YP766539EYO
CSB-EP766539EYO
CSB-BP766539EYO
CSB-MP766539EYO
CSB-EP766539EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 10 kDa chaperonin (groS) CSB-YP766540EYO
CSB-EP766540EYO
CSB-BP766540EYO
CSB-MP766540EYO
CSB-EP766540EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S14 (rpsN) CSB-YP766541EYO
CSB-EP766541EYO
CSB-BP766541EYO
CSB-MP766541EYO
CSB-EP766541EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Leucine--tRNA ligase (leuS), partial CSB-YP766542EYO
CSB-EP766542EYO
CSB-BP766542EYO
CSB-MP766542EYO
CSB-EP766542EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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