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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus GTPase Der (der) CSB-YP766783EYP
CSB-EP766783EYP
CSB-BP766783EYP
CSB-MP766783EYP
CSB-EP766783EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Release factor glutamine methyltransferase (prmC) CSB-YP766784EYP
CSB-EP766784EYP
CSB-BP766784EYP
CSB-MP766784EYP
CSB-EP766784EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b559 subunit alpha (psbE), partial CSB-YP766785EYP1
CSB-EP766785EYP1
CSB-BP766785EYP1
CSB-MP766785EYP1
CSB-EP766785EYP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Queuine tRNA-ribosyltransferase (tgt) CSB-YP766786EYP
CSB-EP766786EYP
CSB-BP766786EYP
CSB-MP766786EYP
CSB-EP766786EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP766787EYP
CSB-EP766787EYP
CSB-BP766787EYP
CSB-MP766787EYP
CSB-EP766787EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Aspartate carbamoyltransferase (pyrB) CSB-YP766788EYP
CSB-EP766788EYP
CSB-BP766788EYP
CSB-MP766788EYP
CSB-EP766788EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP766789EYP
CSB-EP766789EYP
CSB-BP766789EYP
CSB-MP766789EYP
CSB-EP766789EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Adenylyl-sulfate kinase (cysC) CSB-YP766790EYP
CSB-EP766790EYP
CSB-BP766790EYP
CSB-MP766790EYP
CSB-EP766790EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphate acyltransferase (plsX) CSB-YP766791EYP
CSB-EP766791EYP
CSB-BP766791EYP
CSB-MP766791EYP
CSB-EP766791EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP766792EYP
CSB-EP766792EYP
CSB-BP766792EYP
CSB-MP766792EYP
CSB-EP766792EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Alanine--tRNA ligase (alaS), partial CSB-YP766793EYP
CSB-EP766793EYP
CSB-BP766793EYP
CSB-MP766793EYP
CSB-EP766793EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cobalamin synthase (cobS), partial CSB-YP766794MVH1
CSB-EP766794MVH1
CSB-BP766794MVH1
CSB-MP766794MVH1
CSB-EP766794MVH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha-pyrone synthesis polyketide synthase-like Pks11 (pks11) CSB-YP766795MVH
CSB-EP766795MVH
CSB-BP766795MVH
CSB-MP766795MVH
CSB-EP766795MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP766796MVH
CSB-EP766796MVH
CSB-BP766796MVH
CSB-MP766796MVH
CSB-EP766796MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Non-canonical purine NTP pyrophosphatase (HH_1835) CSB-YP766797HCO
CSB-EP766797HCO
CSB-BP766797HCO
CSB-MP766797HCO
CSB-EP766797HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Adenylate kinase (adk) CSB-YP766798HCO
CSB-EP766798HCO
CSB-BP766798HCO
CSB-MP766798HCO
CSB-EP766798HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Glutamyl-tRNA reductase (hemA) CSB-YP766799HCO
CSB-EP766799HCO
CSB-BP766799HCO
CSB-MP766799HCO
CSB-EP766799HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Dephospho-CoA kinase (coaE) CSB-YP766800HCO
CSB-EP766800HCO
CSB-BP766800HCO
CSB-MP766800HCO
CSB-EP766800HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP766801HCO
CSB-EP766801HCO
CSB-BP766801HCO
CSB-MP766801HCO
CSB-EP766801HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP766802HCO1
CSB-EP766802HCO1
CSB-BP766802HCO1
CSB-MP766802HCO1
CSB-EP766802HCO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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