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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nitrosomonas europaea Histidinol-phosphate aminotransferase 2 (hisC2) CSB-YP767724NHH
CSB-EP767724NHH
CSB-BP767724NHH
CSB-MP767724NHH
CSB-EP767724NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP767725NHH
CSB-EP767725NHH
CSB-BP767725NHH
CSB-MP767725NHH
CSB-EP767725NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Bifunctional protein GlmU (glmU) CSB-YP767726NHH
CSB-EP767726NHH
CSB-BP767726NHH
CSB-MP767726NHH
CSB-EP767726NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 50S ribosomal protein L9 (rplI) CSB-YP767727NHH
CSB-EP767727NHH
CSB-BP767727NHH
CSB-MP767727NHH
CSB-EP767727NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP767728NHH
CSB-EP767728NHH
CSB-BP767728NHH
CSB-MP767728NHH
CSB-EP767728NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Probable CRISPR-associated endoribonuclease Cas2 2 (cas22) CSB-YP767729NHH
CSB-EP767729NHH
CSB-BP767729NHH
CSB-MP767729NHH
CSB-EP767729NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP767730NHH
CSB-EP767730NHH
CSB-BP767730NHH
CSB-MP767730NHH
CSB-EP767730NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Probable intracellular septation protein A (NE0055), partial CSB-YP767731NHH1
CSB-EP767731NHH1
CSB-BP767731NHH1
CSB-MP767731NHH1
CSB-EP767731NHH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP767732NHH
CSB-EP767732NHH
CSB-BP767732NHH
CSB-MP767732NHH
CSB-EP767732NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Citrate lyase acyl carrier protein (citD) CSB-YP767733ELW
CSB-EP767733ELW
CSB-BP767733ELW
CSB-MP767733ELW
CSB-EP767733ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 30S ribosomal protein S14 2 (rpsN2) CSB-YP767734ELW
CSB-EP767734ELW
CSB-BP767734ELW
CSB-MP767734ELW
CSB-EP767734ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis DNA mismatch repair protein MutS (mutS), partial CSB-YP767735ELW
CSB-EP767735ELW
CSB-BP767735ELW
CSB-MP767735ELW
CSB-EP767735ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Phosphate acyltransferase (plsX) CSB-YP767736ELW
CSB-EP767736ELW
CSB-BP767736ELW
CSB-MP767736ELW
CSB-EP767736ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP767737ELW
CSB-EP767737ELW
CSB-BP767737ELW
CSB-MP767737ELW
CSB-EP767737ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis UPF0758 protein EF_2926 (EF_2926) CSB-YP767738ELW
CSB-EP767738ELW
CSB-BP767738ELW
CSB-MP767738ELW
CSB-EP767738ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lettuce infectious yellows virus Replicase polyprotein 1a (ORF1a), partial CSB-YP767739LEY
CSB-EP767739LEY
CSB-BP767739LEY
CSB-MP767739LEY
CSB-EP767739LEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lettuce infectious yellows virus Minor capsid protein CSB-YP767740LEY
CSB-EP767740LEY
CSB-BP767740LEY
CSB-MP767740LEY
CSB-EP767740LEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Probable transcriptional regulatory protein EF_2866 (EF_2866) CSB-YP767741ELW
CSB-EP767741ELW
CSB-BP767741ELW
CSB-MP767741ELW
CSB-EP767741ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Initiation-control protein yabA (EF_2760) CSB-YP767742ELW
CSB-EP767742ELW
CSB-BP767742ELW
CSB-MP767742ELW
CSB-EP767742ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 50S ribosomal protein L7/L12 (rplL) CSB-YP767743ELW
CSB-EP767743ELW
CSB-BP767743ELW
CSB-MP767743ELW
CSB-EP767743ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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