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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterococcus faecalis Adapter protein mecA (mecA) CSB-YP767744ELW
CSB-EP767744ELW
CSB-BP767744ELW
CSB-MP767744ELW
CSB-EP767744ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Redox-sensing transcriptional repressor rex 1 (rex1) CSB-YP767745ELW
CSB-EP767745ELW
CSB-BP767745ELW
CSB-MP767745ELW
CSB-EP767745ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis ATP synthase gamma chain (atpG) CSB-YP767746ELW
CSB-EP767746ELW
CSB-BP767746ELW
CSB-MP767746ELW
CSB-EP767746ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Methionine import ATP-binding protein MetN 2 (metN2) CSB-YP767747ELW
CSB-EP767747ELW
CSB-BP767747ELW
CSB-MP767747ELW
CSB-EP767747ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis UPF0223 protein EF_2462 (EF_2462) CSB-YP767748ELW
CSB-EP767748ELW
CSB-BP767748ELW
CSB-MP767748ELW
CSB-EP767748ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis GMP reductase (guaC) CSB-YP767749ELW
CSB-EP767749ELW
CSB-BP767749ELW
CSB-MP767749ELW
CSB-EP767749ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis DNA repair protein recO (recO) CSB-YP767750ELW
CSB-EP767750ELW
CSB-BP767750ELW
CSB-MP767750ELW
CSB-EP767750ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Ribosome-recycling factor (frr) CSB-YP767751ELW
CSB-EP767751ELW
CSB-BP767751ELW
CSB-MP767751ELW
CSB-EP767751ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murid herpesvirus 1 G-protein coupled receptor homolog M33, partial CSB-YP767752MJT1
CSB-EP767752MJT1
CSB-BP767752MJT1
CSB-MP767752MJT1
CSB-EP767752MJT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Capsid protein (ORF3b) CSB-YP767753CIAQ
CSB-EP767753CIAQ
CSB-BP767753CIAQ
CSB-MP767753CIAQ
CSB-EP767753CIAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine coronavirus Nucleoprotein (N) CSB-YP767754MUT
CSB-EP767754MUT
CSB-BP767754MUT
CSB-MP767754MUT
CSB-EP767754MUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Histidine--tRNA ligase (hisS) CSB-YP767755ELW
CSB-EP767755ELW
CSB-BP767755ELW
CSB-MP767755ELW
CSB-EP767755ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Putative membrane protein insertion efficiency factor (EF_1913) CSB-YP767756ELW
CSB-EP767756ELW
CSB-BP767756ELW
CSB-MP767756ELW
CSB-EP767756ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Pantothenate synthetase (panC) CSB-YP767757ELW
CSB-EP767757ELW
CSB-BP767757ELW
CSB-MP767757ELW
CSB-EP767757ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis ADP-dependent (S)-NAD (P)H-hydrate dehydratase (nnrD) CSB-YP767758ELW
CSB-EP767758ELW
CSB-BP767758ELW
CSB-MP767758ELW
CSB-EP767758ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid protein VP4, partial CSB-YP767759RCAM
CSB-EP767759RCAM
CSB-BP767759RCAM
CSB-MP767759RCAM
CSB-EP767759RCAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Southern cowpea mosaic virus Polyprotein P2A (ORF2A), partial CSB-YP767760SIY1
CSB-EP767760SIY1
CSB-BP767760SIY1
CSB-MP767760SIY1
CSB-EP767760SIY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis UPF0154 protein EF_1734 (EF_1734), partial CSB-YP767761ELW
CSB-EP767761ELW
CSB-BP767761ELW
CSB-MP767761ELW
CSB-EP767761ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 30S ribosomal protein S16 (rpsP) CSB-YP767762ELW
CSB-EP767762ELW
CSB-BP767762ELW
CSB-MP767762ELW
CSB-EP767762ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP767763ELW
CSB-EP767763ELW
CSB-BP767763ELW
CSB-MP767763ELW
CSB-EP767763ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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